下载GeneBank文件(.gb)

LOCUS       pAcGP67-C.        9766 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer vector for secreting expressed proteins using 
            the GP64 signal sequence. For other reading frames, use pAcGP67-A or
            pAcGP67-B.
ACCESSION   .
VERSION     .
KEYWORDS    pAcGP67-C.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9766)
  AUTHORS   BD Biosciences
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 9766)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             76..579
                     /gene="PTP"
                     /label=PTP
                     /note="Tyrosine-protein phosphatase from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P24656"
     CDS             2662..3291
                     /gene="LEF-2"
                     /label=LEF-2
                     /note="Primase-associated factor LEF-2 from Autographa 
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     3170..3997
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     promoter        4001..4092
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     sig_peptide     4189..4248
                     /label=GP64 signal sequence
                     /note="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
     misc_feature    4259..4319
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     misc_recomb     4781..6236
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     primer_bind     complement(7147..7163)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7171..7187)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7195..7225)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7240..7261)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7549..8137)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8311..9168)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9169..9273)
                     /label=AmpR promoter
     primer_bind     9747..9763
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"