LOCUS       pAcGP67-C.        9766 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer vector for secreting expressed proteins using 
            the GP64 signal sequence. For other reading frames, use pAcGP67-A or
            pAcGP67-B.
ACCESSION   .
VERSION     .
KEYWORDS    pAcGP67-C.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9766)
  AUTHORS   BD Biosciences
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 9766)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             76..579
                     /gene="PTP"
                     /label=PTP
                     /note="Tyrosine-protein phosphatase from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P24656"
     CDS             2662..3291
                     /gene="LEF-2"
                     /label=LEF-2
                     /note="Primase-associated factor LEF-2 from Autographa 
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     3170..3997
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     promoter        4001..4092
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     sig_peptide     4189..4248
                     /label=GP64 signal sequence
                     /note="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
     misc_feature    4259..4319
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     misc_recomb     4781..6236
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     primer_bind     complement(7147..7163)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7171..7187)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7195..7225)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7240..7261)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7549..8137)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8311..9168)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9169..9273)
                     /label=AmpR promoter
     primer_bind     9747..9763
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagctttact cgtaaagcga gttgaaggat catatttagt tgcgtttatg agataagatt
       61 gaaagcacgt gtaaaatgtt tcccgcgcgt tggcacaact atttacaatg cggccaagtt
      121 ataaaagatt ctaatctgat atgttttaaa acacctttgc ggcccgagtt gtttgcgtac
      181 gtgactagcg aagaagatgt gtggaccgca gaacagatag taaaacaaaa ccctagtatt
      241 ggagcaataa tcgatttaac caacacgtct aaatattatg atggtgtgca ttttttgcgg
      301 gcgggcctgt tatacaaaaa aattcaagta cctggccaga ctttgccgcc tgaaagcata
      361 gttcaagaat ttattgacac ggtaaaagaa tttacagaaa agtgtcccgg catgttggtg
      421 ggcgtgcact gcacacacgg tattaatcgc accggttaca tggtgtgcag atatttaatg
      481 cacaccctgg gtattgcgcc gcaggaagcc atagatagat tcgaaaaagc cagaggtcac
      541 aaaattgaaa gacaaaatta cgttcaagat ttattaattt aattaatatt atttgcattc
      601 tttaacaaat actttatcct attttcaaat tgttgcgctt cttccagcga accaaaacta
      661 tgcttcgctt gctccgttta gcttgtagcc gatcagtggc gttgttccaa tcgacggtag
      721 gattaggccg gatattctcc accacaatgt tggcaacgtt gatgttacgt ttatgctttt
      781 ggttttccac gtacgtcttt tggccggtaa tagccgtaaa cgtagtgccg tcgcgcgtca
      841 cgcacaacac cggatgtttg cgcttgtccg cggggtattg aaccgcgcga tccgacaaat
      901 ccaccacttt ggcaactaaa tcggtgacct gcgcgtcttt tttctgcatt atttcgtctt
      961 tcttttgcat ggtttcctgg aagccggtgt acatgcggtt tagatcagtc atgacgcgcg
     1021 tgacctgcaa atctttggcc tcgatctgct tgtccttgat ggcaacgatg cgttcaataa
     1081 actcttgttt tttaacaagt tcctcggttt tttgcgccac caccgcttgc agcgcgtttg
     1141 tgtgctcggt gaatgtcgca atcagcttag tcaccaactg tttgctctcc tcctcccgtt
     1201 gtttgatcgc gggatcgtac ttgccggtgc agagcacttg aggaattact tcttctaaaa
     1261 gccattcttg taattctatg gcgtaaggca atttggactt cataatcagc tgaatcacgc
     1321 cggatttagt aatgagcact gtatgcggct gcaaatacag cgggtcgccc cttttcacga
     1381 cgctgttaga ggtagggccc ccattttgga tggtctgctc aaataacgat ttgtatttat
     1441 tgtctacatg aacacgtata gctttatcac aaactgtata ttttaaactg ttagcgacgt
     1501 ccttggccac gaaccggacc tgttggtcgc gctctagcac gtaccgcagg ttgaacgtat
     1561 cttctccaaa tttaaattct ccaattttaa cgcgagccat tttgatacac gtgtgtcgat
     1621 tttgcaacaa ctattgtttt ttaacgcaaa ctaaacttat tgtggtaagc aataattaaa
     1681 tatgggggaa catgcgccgc tacaacactc gtcgttatga acgcagacgg cgccggtctc
     1741 ggcgcaagcg gctaaaacgt gttgcgcgtt caacgcggca aacatcgcaa aagccaatag
     1801 tacagttttg atttgcatat taacggcgat tttttaaatt atcttattta ataaatagtt
     1861 atgacgccta caactccccg cccgcgttga ctcgctgcac ctcgagcagt tcgttgacgc
     1921 cttcctccgt gtggccgaac acgtcgagcg ggtggtcgat gaccagcggc gtgccgcacg
     1981 cgacgcacaa gtatctgtac accgaatgat cgtcgggcga aggcacgtcg gcctccaagt
     2041 ggcaatattg gcaaattcga aaatatatac agttgggttg tttgcgcata tctatcgtgg
     2101 cgttgggcat gtacgtccga acgttgattt gcatgcaagc cgaaattaaa tcattgcgat
     2161 tagtgcgatt aaaacgttgt acatcctcgc ttttaatcat gccgtcgatt aaatcgcgca
     2221 atcgagtcaa gtgatcaaag tgtggaataa tgttttcttt gtattcccga gtcaagcgca
     2281 gcgcgtattt taacaaacta gccatcttgt aagttagttt catttaatgc aactttatcc
     2341 aataatatat tatgtatcgc acgtcaagaa ttaacaatgc gcccgttgtc gcatctcaac
     2401 acgactatga tagagatcaa ataaagcgcg aattaaatag cttgcgacgc aacgtgcacg
     2461 atctgtgcac gcgttccggc acgagctttg attgtaataa gtttttacga agcgatgaca
     2521 tgacccccgt agtgacaacg atcacgccca aaagaactgc cgactacaaa attaccgagt
     2581 atgtcggtga cgttaaaact attaagccat ccaatcgacc gttagtcgaa tcaggaccgc
     2641 tggtgcgaga agccgcgaag tatggcgaat gcatcgtata acgtgtggag tccgctcatt
     2701 agagcgtcat gtttagacaa gaaagctaca tatttaattg atcccgatga ttttattgat
     2761 aaattgaccc taactccata cacggtattc tacaatggcg gggttttggt caaaatttcc
     2821 ggactgcgat tgtacatgct gttaacggct ccgcccacta ttaatgaaat taaaaattcc
     2881 aattttaaaa aacgcagcaa gagaaacatt tgtatgaaag aatgcgtaga aggaaagaaa
     2941 aatgtcgtcg acatgctgaa caacaagatt aatatgcctc cgtgtataaa aaaaatattg
     3001 aacgatttga aagaaaacaa tgtaccgcgc ggcggtatgt acaggaagag gtttatacta
     3061 aactgttaca ttgcaaacgt ggtttcgtgt gccaagtgtg aaaaccgatg tttaatcaag
     3121 gctctgacgc atttctacaa ccacgactcc aagtgtgtgg gtgaagtcat gcatctttta
     3181 atcaaatccc aagatgtgta taaaccacca aactgccaaa aaatgaaaac tgtcgacaag
     3241 ctctgtccgt ttgctggcaa ctgcaagggt ctcaatccta tttgtaatta ttgaataata
     3301 aaacaattat aaatgctaaa tttgtttttt attaacgata caaaccaaac gcaacaagaa
     3361 catttgtagt attatctata attgaaaacg cgtagttata atcgctgagg taatatttaa
     3421 aatcattttc aaatgattca cagttaattt gcgacaatat aattttattt tcacataaac
     3481 tagacgcctt gtcgtcttct tcttcgtatt ccttctcttt ttcatttttc tcctcataaa
     3541 aattaacata gttattatcg tatccatata tgtatctatc gtatagagta aattttttgt
     3601 tgtcataaat atatatgtct tttttaatgg ggtgtatagt accgctgcgc atagtttttc
     3661 tgtaatttac aacagtgcta ttttctggta gttcttcgga gtgtgttgct ttaattatta
     3721 aatttatata atcaatgaat ttgggatcgt cggttttgta caatatgttg ccggcatagt
     3781 acgcagcttc ttctagttca attacaccat tttttagcag caccggatta acataacttt
     3841 ccaaaatgtt gtacgaaccg ttaaacaaaa acagttcacc tcccttttct atactattgt
     3901 ctgcgagcag ttgtttgttg ttaaaaataa cagccattgt aatgagacgc acaaactaat
     3961 atcacaaact ggaaatgtct atcaatatat agttgctgat atcatggaga taattaaaat
     4021 gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa
     4081 aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg atctatgcta
     4141 ctagtaaatc agtcacacca aggcttcaat aaggaacaca caagcaagat ggtaagcgct
     4201 attgttttat atgtgctttt ggcggcggcg gcgcattctg cctttgcggc ggatctatgg
     4261 atcccgggcc atgggaattc cggagcggcc gctgcagatc tgatcctttc ctgggacccg
     4321 gcaagaacca aaaactcact ctcttcaagg aaatccgtaa tgttaaaccc gacacgatga
     4381 agcttgtcgt tggatggaaa ggaaaagagt tctacaggga aacttggacc cgcttcatgg
     4441 aagacagctt ccccattgtt aacgaccaag aagtgatgga tgttttcctt gttgtcaaca
     4501 tgcgtcccac tagacccaac cgttgttaca aattcctggc ccaacacgct ctgcgttgcg
     4561 accccgacta tgtacctcat gacgtgatta ggatcgtcga gccttcatgg gtgggcagca
     4621 acaacgagta ccgcatcagc ctggctaaga agggcggcgg ctgcccaata atgaaccttc
     4681 actctgagta caccaactcg ttcgaacagt tcatcgatcg tgtcatctgg gagaacttct
     4741 acaagcccat cgtttacatc ggtaccgact ctgctgaaga ggaggaaatt ctccttgaag
     4801 tttccctggt gttcaaagta aaggagtttg caccagacgc acctctgttc actggtccgg
     4861 cgtattaaaa cacgatacat tgttattagt acatttatta agcgctagat tctgtgcgtt
     4921 gttgatttac agacaattgt tgtacgtatt ttaataattc attaaattta taatctttag
     4981 ggtggtatgt tagagcgaaa atcaaatgat tttcagcgtc tttatatctg aatttaaata
     5041 ttaaatcctc aatagatttg taaaataggt ttcgattagt ttcaaacaag ggttgttttt
     5101 ccgaaccgat ggctggacta tctaatggat tttcgctcaa cgccacaaaa cttgccaaat
     5161 cttgtagcag caatctagct ttgtcgatat tcgtttgtgt tttgttttgt aataaaggtt
     5221 cgacgtcgtt caaaatatta tgcgcttttg tatttctttc atcactgtcg ttagtgtaca
     5281 attgactcga cgtaaacacg ttaaataaag cttggacata tttaacatcg ggcgtgttag
     5341 ctttattagg ccgattatcg tcgtcgtccc aaccctcgtc gttagaagtt gcttccgaag
     5401 acgattttgc catagccaca cgacgcctat taattgtgtc ggctaacacg tccgcgatca
     5461 aatttgtagt tgagcttttt ggaattattt ctgattgcgg gcgtttttgg gcgggtttca
     5521 atctaactgt gcccgatttt aattcagaca acacgttaga aagcgatggt gcaggcggtg
     5581 gtaacatttc agacggcaaa tctactaatg gcggcggtgg tggagctgat gataaatcta
     5641 ccatcggtgg aggcgcaggc ggggctggcg gcggaggcgg aggcggaggt ggtggcggtg
     5701 atgcagacgg cggtttaggc tcaaatgtct ctttaggcaa cacagtcggc acctcaacta
     5761 ttgtactggt ttcgggcgcc gtttttggtt tgaccggtct gagacgagtg cgattttttt
     5821 cgtttctaat agcttccaac aattgttgtc tgtcgtctaa aggtgcagcg ggttgaggtt
     5881 ccgtcggcat tggtggagcg ggcggcaatt cagacatcga tggtggtggt ggtggtggag
     5941 gcgctggaat gttaggcacg ggagaaggtg gtggcggcgg tgccgccggt ataatttgtt
     6001 ctggtttagt ttgttcgcgc acgattgtgg gcaccggcgc aggcgccgct ggctgcacaa
     6061 cggaaggtcg tctgcttcga ggcagcgctt ggggtggtgg caattcaata ttataattgg
     6121 aatacaaatc gtaaaaatct gctataagca ttgtaatttc gctatcgttt accgtgccga
     6181 tatttaacaa ccgctcaatg taagcaattg tattgtaaag agattgtctc aagctccgca
     6241 cgccgataac aagccttttc atttttacta cagcattgta gtggcgagac acttcgctgt
     6301 cgtcgacgta catgtatgct ttgttgtcaa aaacgtcgtt ggcaagcttt aaaatattta
     6361 aaagaacatc tctgttcagc accactgtgt tgtcgtaaat gttgtttttg ataatttgcg
     6421 cttccgcagt atcgacacgt tcaaaaaatt gatgcgcatc aattttgttg ttcctattat
     6481 tgaataaata agattgtaca gattcatatc tacgattcgt catggccacc acaaatgcta
     6541 cgctgcaaac gctggtacaa ttttacgaaa actgcaaaaa cgtcaaaact cggtataaaa
     6601 taatcaacgg gcgctttggc aaaatatcta ttttatcgca caagcccact agcaaattgt
     6661 atttgcagaa aacaatttcg gcgcacaatt ttaacgctga cgaaataaaa gttcaccagt
     6721 taatgagcga ccacccaaat tttataaaaa tctattttaa tcacggttcc atcaacaacc
     6781 aagtgatcgt gatggactac attgactgtc ccgatttatt tgaaacacta caaattaaag
     6841 gcgagctttc gtaccaactt gttagcaata ttattagaca gctgtgtgaa gcgctcaacg
     6901 atttgcacaa gcacaatttc atacacaacg acataaaact cgaaaatgtc ttatatttcg
     6961 aagcacttga tcgcgtgtat gtttgcgatt acggattgtg caaacacgaa aactcactta
     7021 gcgtgcacga cggcacgttg gagtatttta gtccggaaaa aattcgacac acaactatgc
     7081 acgtttcgtt tgactggtac gcggcgtgtt aacatacaag ttgctaaccg gcggttcgta
     7141 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     7201 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     7261 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     7321 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     7381 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     7441 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     7501 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     7561 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     7621 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     7681 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     7741 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     7801 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     7861 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     7921 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     7981 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     8041 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     8101 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     8161 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     8221 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     8281 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     8341 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     8401 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     8461 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     8521 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     8581 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     8641 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     8701 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     8761 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     8821 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     8881 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     8941 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     9001 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     9061 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     9121 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     9181 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     9241 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     9301 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     9361 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     9421 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     9481 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
     9541 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     9601 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     9661 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     9721 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgcc
//
