- 载体名称:
- pBABE-Puro
- 载体抗性:
- Ampicillin
- 载体长度:
- 5169 bp
- 载体类型:
- Viral Expression & Packaging Vectors
- 复制子:
- ori
- 载体来源:
- Morgenstern JP, Land H.
- 筛选标记:
- Puromycin
- 拷贝数:
- High copy number
- 克隆方法:
- Restriction Enzyme
- 5'引物:
- pBABE 5'
- 3'引物:
- pBABE 3'
pBABE-Puro 载体载体图谱
pBABE-Puro 载体载体序列
LOCUS pBABE-Puro. 5169 bp DNA circular SYN 01-JAN-1980 DEFINITION Retroviral expression vector derived from the Moloney murine leukemia virus. Contains a puromycin resistance marker. ACCESSION . VERSION . KEYWORDS pBABE-Puro. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5169) AUTHORS Morgenstern JP, Land H. TITLE Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line. JOURNAL Nucleic Acids Res. 1990;18:3587-96. PUBMED 2194165 REFERENCE 2 (bases 1 to 5169) AUTHORS Cell Biolabs TITLE Direct Submission REFERENCE 3 (bases 1 to 5169) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic Acids Res."; date: "1990"; volume: "18"; pages: "3587-96" COMMENT SGRef: number: 2; type: "Journal Article" COMMENT Strong expression is driven by the viral promoter in the 5' LTR. The commercially provided sequence was modified to include the splice donor mutation. FEATURES Location/Qualifiers source 1..5169 /mol_type="other DNA" /organism="synthetic DNA construct" LTR 8..477 /label=LTR /note="long terminal repeat from Moloney murine leukemia virus" misc_feature 541 /label=splice donor mutation /note="splice donor mutation" /note="T-to-C mutation that blocks RNA splicing" misc_feature 542..741 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 934..1350 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" misc_feature 1355..1402 /label=MCS /note="MCS" /note="multiple cloning site" promoter 1413..1742 /label=SV40 promoter /note="SV40 enhancer and early promoter" CDS 1752..2348 /label=PuroR /note="puromycin N-acetyltransferase" LTR 2450..3043 /label=LTR /note="long terminal repeat from Moloney murine leukemia virus" rep_origin complement(3415..4003) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4184..5041) /label=AmpR /note="beta-lactamase" promoter complement(5042..5146) /label=AmpR promoter
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