LOCUS       pBABE-Puro.        5169 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Retroviral expression vector derived from the Moloney murine 
            leukemia virus. Contains a puromycin resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    pBABE-Puro.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Morgenstern JP, Land H.
  TITLE     Advanced mammalian gene transfer: high titre retroviral vectors with
            multiple drug selection markers and a complementary helper-free 
            packaging cell line.
  JOURNAL   Nucleic Acids Res. 1990;18:3587-96.
  PUBMED    2194165
REFERENCE   2  (bases 1 to 5169)
  AUTHORS   Cell Biolabs
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5169)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1990"; volume: "18"; pages: "3587-96"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     Strong expression is driven by the viral promoter in the 5' LTR. The
            commercially provided sequence was modified to include the splice 
            donor mutation.
FEATURES             Location/Qualifiers
     source          1..5169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..477
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     misc_feature    541
                     /label=splice donor mutation
                     /note="splice donor mutation"
                     /note="T-to-C mutation that blocks RNA splicing"
     misc_feature    542..741
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             934..1350
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     misc_feature    1355..1402
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        1413..1742
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             1752..2348
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     LTR             2450..3043
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     rep_origin      complement(3415..4003)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4184..5041)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5042..5146)
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt
      901 ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt
      961 ttgaccttag atcactggaa agatgtcgag cggctcgctc acaaccagtc ggtagatgtc
     1021 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg
     1081 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca
     1141 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct
     1201 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttctt
     1261 ccatccgcgc cgtctctccc ccttgaacct cctctttcga ccccgcctca atcctccctt
     1321 tatccagccc tcactccttc tctaggcgcc ggccggatcc cagtgtggtg gtacgtagga
     1381 attcgccagc acagtggtcg accctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     1441 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     1501 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     1561 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     1621 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     1681 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     1741 aaaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     1801 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     1861 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     1921 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     1981 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     2041 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     2101 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     2161 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     2221 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     2281 ccgtcaccgc cgacgtcgag tgcccgaagg accgcgcgac ctggtgcatg acccgcaagc
     2341 ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc
     2401 atgcatcgat aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc
     2461 ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaaata
     2521 cataactgag aatagagaag ttcagatcaa ggtcaggaac agatggaaca gctgaatatg
     2581 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg
     2641 gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
     2701 ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta gagaaccatc
     2761 agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca
     2821 atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc
     2881 cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt
     2941 atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt
     3001 ctcctctgag tgattgacta cccgtcagcg ggggtctttc atttccgact tgtggtctcg
     3061 ctgccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt cttcacatgc
     3121 agcatgtatc aaaattaatt tggttttttt tcttaagtat ttacattaaa tggccatagt
     3181 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggc gctcttccgc
     3241 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     3301 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     3361 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     3421 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     3481 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     3541 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     3601 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     3661 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     3721 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     3781 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     3841 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     3901 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     3961 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     4021 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     4081 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt tgcggccgca
     4141 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     4201 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     4261 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     4321 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     4381 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     4441 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     4501 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     4561 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     4621 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     4681 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     4741 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
     4801 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     4861 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     4921 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     4981 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     5041 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     5101 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     5161 aagtgccac
//
