- 载体名称:
- pALH122
- 载体抗性:
- Chloramphenicol
- 载体长度:
- 12248 bp
- 载体类型:
- Reporter vector
- 复制子:
- p15A ori
- 载体来源:
- Honeyman AL, Cote CK, Curtiss R III.
pALH122 载体载体图谱
pALH122 载体载体序列
LOCUS 40924_4359 12248 bp DNA circular SYN 17-DEC-2018 DEFINITION Reporter vector pALH122, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12248) AUTHORS Honeyman AL, Cote CK, Curtiss R III. TITLE Construction of transcriptional and translational lacZ gene reporter plasmids for use in Streptococcus mutans JOURNAL J. Microbiol. Methods 49 (2), 163-171 (2002) PUBMED 11830302 REFERENCE 2 (bases 1 to 12248) AUTHORS Honeyman AL, Cote CK, Curtiss R III. TITLE Direct Submission JOURNAL Submitted (06-AUG-2001) Department of Medical Microbiology and Immunology, University of South Florida, 12901 Bruce B. Downs Blvd., Tampa, FL 33612, USA REFERENCE 3 (bases 1 to 12248) TITLE Direct Submission REFERENCE 4 (bases 1 to 12248) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J. Microbiol. Methods"; date: "2002"; volume: "49"; issue: "2"; pages: "163-171" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (06-AUG-2001) Department of Medical Microbiology and Immunology, University of South Florida, 12901 Bruce B. Downs Blvd., Tampa, FL 33612, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..12248 /mol_type="other DNA" /organism="synthetic DNA construct" CDS complement(655..3726) /label=lacZ /note="beta-galactosidase" regulatory complement(3735..3741) /label=lacZ modified /note="lacZ modified" /regulatory_class="ribosome_binding_site" misc_feature complement(3741..3743) /label=stop codon /note="stop codon" misc_feature complement(3745..3747) /label=stop codon /note="stop codon" misc_feature complement(3749..3751) /label=stop codon /note="stop codon" CDS complement(5468..6124) /label=CmR /note="chloramphenicol acetyltransferase" promoter complement(6125..6227) /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" rep_origin complement(6753..7298) /direction=LEFT /label=p15A ori /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin." CDS 9260..9973 /codon_start=1 /gene="rep" /product="replication protein" /label=rep /note="derived from pVA380-1; required for Gram positive hosts" /protein_id="AAN02499.1" /translation="MKYAYQAELVVNEAMKRYPKGRFLFLTLTIKNISGEKLNKSISEI GRAFNRLMKYKKVDKNVIGYLRATEVTYSTEHENYHPHLHVLLFVKSSYFTGNNTNYIS QEEWTKLWAKAMKLDYTPVVDIRTVKAHKRKNLKSAIIETAKYPVKPFDVDTEDVTLFS EMVKERITEDLTNGLHRKRQIGFGKLFKKIKAELALDDVEEGNLVQTGAEESAESTGRE IVAFWNWDRKNYFVR" gene 9260..9973 /gene="rep" /label=rep CDS complement(10601..11335) /gene="ermBP" /label=ermBP /note="rRNA adenine N-6-methyltransferase from Enterococcus faecalis. Accession#: P0A4D5"
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