- 载体名称:
- pCold-SUMO
- 载体抗性:
- Ampicillin
- 载体长度:
- 4695 bp
- 载体类型:
- Protein expression
- 复制子:
- ori
- 宿主:
- E. coli
- 启动子:
- cspA
pCold-SUMO 载体载体图谱
pCold-SUMO 载体载体序列
LOCUS 62056_7860 4695 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4695) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..4695 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 15..81 /label=cspA promoter /note="promoter of the E. coli cold shock protein cspA gene (Mitta et al., 1997)" protein_bind 84..100 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." 5'UTR 122..255 /label=cspA 5'UTR /note="5'UTR of the E. coli cold shock protein cspA gene (Mitta et al., 1997)" CDS 256..270 /codon_start=1 /label=TEE /note="translation enhancing element for E. coli (Qing et al., 2004)" /translation="MNHKV" CDS 271..288 /codon_start=1 /label=6xHis /note="6xHis affinity tag" /translation="HHHHHH" CDS 289..300 /codon_start=1 /label=Factor Xa site /note="Factor Xa recognition and cleavage site" /translation="IEGR" CDS 319..612 /codon_start=1 /label=SUMO /note="cleavable ubiquitin-like protein tag" /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG" 3'UTR 656..800 /label=cspA 3'UTR /note="3'UTR of the E. coli cold shock protein cspA gene (Mitta et al., 1997)" rep_origin complement(999..1454) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 1554..1658 /label=AmpR promoter CDS 1659..2516 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 2690..3278 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind complement(3416..3437) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." CDS complement(3453..4532) /codon_start=1 /label=lacI /note="lac repressor" /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" promoter complement(4533..4610) /label=lacI promoter
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