- 载体名称:
- pFC16A HaloTag CMVd2
- 载体抗性:
- Ampicillin
- 载体长度:
- 4134 bp
- 载体类型:
- Mammalian Expression Vectors
- 复制子:
- ori
- 载体来源:
- Promega
- 拷贝数:
- High copy number
- 启动子:
- CMVd2
pFC16A HaloTag CMVd2 载体载体图谱
pFC16A HaloTag CMVd2 载体载体序列
LOCUS 40924_19801 4134 bp DNA circular SYN 01-JAN-1980 DEFINITION Flexi(R) vector with an ampicillin resistance marker, for mammalian expression from a moderately compromised CMV promoter of a protein with a cleavable C-terminal HaloTag(R). ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4134) AUTHORS Promega TITLE Direct Submission REFERENCE 2 (bases 1 to 4134) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" COMMENT Subclone a coding sequence between the SgfI/AsiSI and EcoICRI/Eco53kI sites to remove the lethal barnase gene. FEATURES Location/Qualifiers source 1..4134 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 1..52 /label=CMVd2 promoter /note="truncated human cytomegalovirus (CMV) immediate early promoter that yields moderately reduced expression relative to the full-length promoter" promoter 84..102 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter 108..126 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" CDS 165..497 /codon_start=1 /label=barnase /note="ribonuclease from Bacillus amyloliquefaciens" /translation="MAQVINTFDGVADYLQTYHKLPDNYITKSEAQALGWVASKGNLAD VAPGKSIGGDIFSNREGKLPGKSGRTWREADINYTSGFRNSDRILYSSDWLIYKTTDHY QTFTKIR" CDS 540..560 /codon_start=1 /label=TEV site /note="tobacco etch virus (TEV) protease recognition and cleavage site" /translation="EDLYFQS" CDS 573..1460 /codon_start=1 /label=HaloTag(R) /note="modified bacterial dehalogenase that forms covalent bonds with chloroalkane derivatives" /translation="SEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI GSEIARWLSTLEISG" polyA_signal complement(1603..1724) /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" promoter 1971..2075 /label=AmpR promoter CDS 2076..2933 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin complement(3094..3682) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" misc_feature 3798..4081 /label=cer region /note="ColE1-derived recombination site that helps to maintain plasmids as monomers"
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