纽普生物

pCDFDuet™-1载体

载体基本信息
载体名称 pCDFDuet™-1 载体抗性 Spectinomycin/Streptomycin
载体长度 3781 bp 载体类型 pET & Duet Vectors (Novagen)
载体来源 Novagen (EMD Millipore) 拷贝数 High copy number

pCDFDuet™-1载体质粒图谱

pCDFDuet™-1载体质粒图谱

pCDFDuet™-1载体载体序列

LOCUS       Exported                3781 bp ds-DNA    circular SYN 27-12-2012
DEFINITION  Bacterial vector with a CloDF13 (CDF) origin for the co-expression 
            of two genes.
ACCESSION   .
VERSION     .
KEYWORDS    pCDFDuet-1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
  JOURNAL   Exported 2017/2/28 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     protein_bind    3..27
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             58..63
                     /note="ribosome binding site"
     misc_feature    69..168
                     /note="MCS-1"
                     /note="multiple cloning site 1"
     CDS             71..73
                     /codon_start=1
                     /note="ATG"
                     /translation="M"
     CDS             83..100
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     promoter        214..232
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    233..257
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             286..291
                     /note="ribosome binding site"
     misc_feature    297..438
                     /note="MCS-2"
                     /note="multiple cloning site 2"
     CDS             300..302
                     /codon_start=1
                     /product="start codon"
                     /note="ATG"
                     /translation="M"
     CDS             366..410
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /note="S-Tag"
                     /translation="KETAAAKFERQHMDS"
     terminator      462..509
                     /note="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(680..1471)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase"
                     /note="SmR"
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1472..1563)
                     /gene="bla"
                     /note="AmpR promoter"
     rep_origin      complement(1611..2349)
                     /direction=LEFT
                     /note="CloDF13 ori"
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     CDS             complement(2559..3641)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /note="lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(3642..3719)
                     /gene="lacI"
                     /note="lacI promoter"
                     /note="
                     "
     promoter        3765..2
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
       61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag
      121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag
      181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt
      241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata
      301 tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt
      361 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg
      421 cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg
      481 cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg
      541 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt
      601 aacgaccctg ccctgaaccg acgaccgggt catcgtggcc ggatcttgcg gcccctcggc
      661 ttgaacgaat tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt
      721 ggacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat
      781 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc
      841 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg
      901 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg
      961 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct
     1021 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca
     1081 gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt
     1141 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa
     1201 caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca
     1261 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca
     1321 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta
     1381 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag
     1441 tcgatacttc ggcgatcacc gcttccctca tactcttcct ttttcaatat tattgaagca
     1501 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     1561 aaatagctag ctcactcggt cgctacgctc cgggcgtgag actgcggcgg gcgctgcgga
     1621 cacatacaaa gttacccaca gattccgtgg ataagcaggg gactaacatg tgaggcaaaa
     1681 cagcagggcc gcgccggtgg cgtttttcca taggctccgc cctcctgcca gagttcacat
     1741 aaacagacgc ttttccggtg catctgtggg agccgtgagg ctcaaccatg aatctgacag
     1801 tacgggcgaa acccgacagg acttaaagat ccccaccgtt tccggcgggt cgctccctct
     1861 tgcgctctcc tgttccgacc ctgccgttta ccggatacct gttccgcctt tctcccttac
     1921 gggaagtgtg gcgctttctc atagctcaca cactggtatc tcggctcggt gtaggtcgtt
     1981 cgctccaagc tgggctgtaa gcaagaactc cccgttcagc ccgactgctg cgccttatcc
     2041 ggtaactgtt cacttgagtc caacccggaa aagcacggta aaacgccact ggcagcagcc
     2101 attggtaact gggagttcgc agaggatttg tttagctaaa cacgcggttg ctcttgaagt
     2161 gtgcgccaaa gtccggctac actggaagga cagatttggt tgctgtgctc tgcgaaagcc
     2221 agttaccacg gttaagcagt tccccaactg acttaacctt cgatcaaacc acctccccag
     2281 gtggtttttt cgtttacagg gcaaaagatt acgcgcagaa aaaaaggatc tcaagaagat
     2341 cctttgatct tttctactga accgctctag atttcagtgc aatttatctc ttcaaatgta
     2401 gcacctgaag tcagccccat acgatataag ttgtaattct catgttagtc atgccccgcg
     2461 cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga gatcccggtg
     2521 cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct ttccagtcgg
     2581 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     2641 gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag ctgattgccc
     2701 ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg
     2761 cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc ttcggtatcg
     2821 tcgtatccca ctaccgagat gtccgcacca acgcgcagcc cggactcggt aatggcgcgc
     2881 attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac gatgccctca
     2941 ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc
     3001 gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag acgcagacgc
     3061 gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc caatgcgacc
     3121 agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact gttgatgggt
     3181 gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc ttccacagca
     3241 atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg ttgcgcgaga
     3301 agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat cgacaccacc
     3361 acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg
     3421 tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc cgccagttgt
     3481 tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac tttttcccgc
     3541 gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg ataagagaca
     3601 ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac cctgaattga
     3661 ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc gatggtgtcc
     3721 gggatctcga cgctctccct tatgcgactc ctgcattagg aaattaatac gactcactat
     3781 a
//

pCDFDuet™-1载体载体特征

一级属性 二级属性 属性值 起始位置
常规长度3781
常规描述Bacterial vector with a CloDF13 (CDF) origin for the co-expression of two genes.
常规是否环形1
常规作者Novagen (EMD Millipore)
常规注释
来源实验宿主Escherichia coli1..3781
来源分子类型other DNA1..3781
来源种属synthetic DNA construct1..3781
蛋白结合位点嵌合范围lac repressor encoded by lacI3..27
蛋白结合位点备注lac operator,The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).3..27
核糖体结合位点备注ribosome binding site58..63
其它特征区备注MCS-1,multiple cloning site 169..168
编码区翻译起始位点171..73
编码区备注ATG71..73
编码区翻译序列M71..73
编码区翻译起始位点183..100
编码区备注6xHis83..100
编码区产物6xHis affinity tag83..100
编码区翻译序列HHHHHH83..100
启动子备注T7 promoter,promoter for bacteriophage T7 RNA polymerase214..232
蛋白结合位点嵌合范围lac repressor encoded by lacI233..257
蛋白结合位点备注lac operator,The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).233..257
核糖体结合位点备注ribosome binding site286..291
其它特征区备注MCS-2,multiple cloning site 2297..438
编码区翻译起始位点1300..302
编码区备注ATG300..302
编码区产物start codon300..302
编码区翻译序列M300..302
编码区翻译起始位点1366..410
编码区备注S-Tag366..410
编码区产物affinity and epitope tag derived from pancreatic ribonuclease A366..410
编码区翻译序列KETAAAKFERQHMDS366..410
终止子备注T7 terminator,transcription terminator for bacteriophage T7 RNA polymerase462..509
编码区翻译起始位点1680..1471
编码区基因aadA680..1471
编码区备注SmR,confers resistance to spectinomycin and streptomycin680..1471
编码区产物aminoglycoside adenylyltransferase680..1471
编码区翻译序列MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK680..1471
启动子基因bla1472..1563
启动子备注AmpR promoter1472..1563
复制起点方向LEFT1611..2349
复制起点备注CloDF13 ori,Plasmids containing the CloDF13 (CDF) origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins.1611..2349
编码区翻译起始位点12559..3641
编码区基因lacI2559..3641
编码区备注lacI,The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).2559..3641
编码区产物lac repressor2559..3641
编码区翻译序列MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ2559..3641
启动子基因lacI3642..3719
启动子备注lacI promoter, 3642..3719
启动子备注T7 promoter,promoter for bacteriophage T7 RNA polymerase2..3765

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