- 载体名称:
- pCDFDuet-1
- 载体抗性:
- Streptomycin
- 载体长度:
- 3781 bp
- 载体类型:
- pET & Duet Vectors (Novagen)
- 复制子:
- CloDF13 ori
- 载体来源:
- Novagen (EMD Millipore)
- 拷贝数:
- High copy number
pCDFDuet-1 载体载体图谱
pCDFDuet-1 载体载体序列
LOCUS pCDFDuet-1. 3781 bp DNA circular SYN 01-JAN-1980 DEFINITION Bacterial vector with a CloDF13 (CDF) origin for the co-expression of two genes. ACCESSION . VERSION . KEYWORDS pCDFDuet-1. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3781) AUTHORS Novagen (EMD Millipore) TITLE Direct Submission REFERENCE 2 (bases 1 to 3781) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..3781 /mol_type="other DNA" /organism="synthetic DNA construct" protein_bind 3..27 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 42..64 /label=RBS /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 71..73 /codon_start=1 /label=ATG /note="ATG" /translation="M" CDS 83..100 /label=6xHis /note="6xHis affinity tag" promoter 214..232 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 233..257 /label=lac repressor encoded by lacI binding site /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 286..291 /note="ribosome binding site" CDS 300..302 /codon_start=1 /product="start codon" /label=start codon /note="ATG" /translation="M" CDS 366..410 /label=S-Tag /note="affinity and epitope tag derived from pancreatic ribonuclease A" terminator 462..509 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" CDS complement(683..1471) /label=SmR /note="aminoglycoside adenylyltransferase (Murphy, 1985)" promoter complement(1472..1563) /label=AmpR promoter rep_origin complement(1611..2349) /direction=LEFT /label=CloDF13 ori /note="Plasmids containing the CloDF13 (CDF) origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins." protein_bind complement(2525..2546) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." CDS complement(2562..3641) /label=lacI /note="lac repressor" promoter complement(3642..3719) /label=lacI promoter
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