载体基本信息 | |||
载体名称 | pET-50b(+) | 载体抗性 | Kanamycin |
---|---|---|---|
载体长度 | 6733 bp | 载体类型 | pET & Duet Vectors (Novagen) |
载体来源 | Novagen (EMD Millipore) | 拷贝数 | High copy number |
pET-50b(+)载体质粒图谱
pET-50b(+)载体载体序列
LOCUS Exported 6733 bp ds-DNA circular SYN 03-12-2013 DEFINITION Bacterial vector for expressing proteins with a cleavable N-terminal NusA tag and a cleavable C-terminal S-tag. ACCESSION . VERSION . KEYWORDS pET-50b(+) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6733) AUTHORS Novagen (EMD Millipore) TITLE Direct Submission JOURNAL Exported 2017/2/28 from SnapGene 2.3.2 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6733 /organism="synthetic DNA construct" /lab_host="Escherichia coli" /mol_type="other DNA" terminator 26..73 /note="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" CDS complement(168..212) /codon_start=1 /product="affinity and epitope tag derived from pancreatic ribonuclease A" /note="S-Tag" /translation="KETAAAKFERQHMDS" CDS complement(213..230) /codon_start=1 /product="thrombin recognition and cleavage site" /note="thrombin site" /translation="LVPRGS" misc_feature 234..308 /note="MCS" /note="multiple cloning site" CDS complement(309..332) /codon_start=1 /product="recognition and cleavage site for human rhinovirus 3C protease" /note="HRV 3C site" /translation="LEVLFQGP" CDS complement(342..359) /codon_start=1 /product="6xHis affinity tag" /note="6xHis" /translation="HHHHHH" CDS complement(375..1859) /codon_start=1 /gene="nusA" /product="transcription elongation protein (N utilization substance protein A)" /note="NusA" /note="highly soluble in E. coli" /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEIDV RVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTFDR ITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEAVIL REDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGEEVIE IKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLWDDNPA QFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGWELNVMT VDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKELLEIEGLD EPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAARGVCTLED LAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA" CDS complement(1866..1883) /codon_start=1 /product="6xHis affinity tag" /note="6xHis" /translation="HHHHHH" CDS complement(1893..1895) /codon_start=1 /product="start codon" /note="ATG" /translation="M" RBS 1904..1909 /note="ribosome binding site" protein_bind 1940..1964 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1965..1983) /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" promoter 2296..2373 /gene="lacI" /note="lacI promoter" /note=" " CDS 2374..3456 /codon_start=1 /gene="lacI" /product="lac repressor" /note="lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" CDS 4028..4219 /codon_start=1 /gene="rop" /product="Rop protein" /note="rop" /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA DELYRSCLARFGDDGENL" rep_origin complement(4649..5237) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS 5359..6174 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /note="KanR" /note="confers resistance to kanamycin" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" rep_origin complement(6267..6722) /direction=LEFT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" ORIGIN 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 121 tgtttagcag cctaggttaa ttaagcctcg agagcagcag aagtagagct gtccatgtgc 181 tggcgttcga atttagcagc agcggtttct ttactaccgc gtggcaccag agcgagctct 241 gcggccgcaa gcttgtcgac ggacgtcggg cgcgccaagg cctgtacaga attcggatcc 301 tggtacccgg gtccctgaaa gaggacttca agagccgcgg agtgatggtg atggtgatga 361 ccagaaccac tagtcgcttc gtcaccgaac cagcaaatat tacgggcagc cataatcagt 421 gctccggctt tttcgtcggt caacccttcg atatcagcca gatcatcaat gccctgttcg 481 gcgagatctt ccagcgtaca aacgccacgg gcggccagtt tgaatgccaa atcacgatct 541 accccttcaa ggttcagcag atcgtcagcc ggtttgttat caccgaggct ttcttcctgg 601 gcctgtgcaa tggtggccag tgcattttta gcacgctcgc gcagtgcttc aacggtcggc 661 tcatcaaggc cttcgatttc caacagctct ttcatcggca cataggccaa ttcttccagc 721 gtcgagaagc cttcttctac cagaacagtc gcgaagtctt cgtcgatgtc gagatatttg 781 gtgaaggtgt cgatcgctgc gtgcgcttcc gcctgatgct tagcttgcag gtcgtcaacg 841 gtcatcacgt tgagttccca accgctcagt tgcgaagcca gacgcacgtt ctgaccgtta 901 cggccaatcg cctgcgccag attaccggct tcaacggcga tgtccatggt gtgtttatct 961 tcatccacca cgatagaagc aacgtctgcc ggtgccattg cgttaatcac gaactgcgcc 1021 gggttatcat cccacaggac gatatcgata cgctcgccac ccagttcagt agacaccgcc 1081 tgaacacgcg cgccacgcat acctacgcaa gcacctaccg gatcgatacg tttatcgttg 1141 gttttcaccg cgattttcgc acgagaaccc ggatcgcgag ccgctgcttt aatttcaatc 1201 acttcttcgc cgatttctgg cacttcaata cggaacagtt cgatcagcat ttccggcttg 1261 gaacgagtga cgaacagttg cgcgccacgc gcttccgggc gaacggaata gagcacgcca 1321 cgaacgcggt cgccagggcg gaagttttca cgcggcagca tatcttcgcg caggatcacg 1381 gcttcagcgt tgttgcccag atccagagag atgttgtcgc ggtttacttt tttcaccacg 1441 ccggtgatga tttcaccttc gtgttcacgg aactgatcaa ccaccatcgc acgttcggct 1501 tcacgcactt tctgcacgat aacctgtttt gccgtctggg tagtgatacg gtcaaaggta 1561 acagactcaa tctgatcttc aacgtaatcg cccaggttca ggctttcatc ttcataacgt 1621 gcggcttcaa gggtgatttc cttggtcggc tgggtgactt catcaacaac taaccagcga 1681 cggaaagtgt caaaatcacc gcttttgcga tcgatctgta cgcggacgtc gatctcttgt 1741 tcatattttt tctttgttgc tgtcgccagc gcgctttcca atgcttcgaa aatcttctcg 1801 cgaggtagcg ccttttcatt ggatacggct tcaactacag ccaaaatttc tttgttcata 1861 ctagaatggt gatggtggtg atggctgctg cccatatgta tatctccttc ttaaagttaa 1921 acaaaattat ttctagaggg gaattgttat ccgctcacaa ttcccctata gtgagtcgta 1981 ttaatttcgc gggatcgaga tcgatctcga tcctctacgc cggacgcatc gtggccggca 2041 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag 2101 atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc 2161 ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg 2221 tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag 2281 agcgtcgaga tcccggacac catcgaatgg cgcaaaacct ttcgcggtat ggcatgatag 2341 cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc 2401 gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac 2461 gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc 2521 aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc 2581 agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa 2641 ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg 2701 gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat 2761 gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat 2821 gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg tacgcgactg 2881 ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta 2941 agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa 3001 attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc 3061 atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg 3121 gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggacatctcg 3181 gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt aaccaccatc 3241 aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag 3301 ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc 3361 ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg 3421 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtaagtta 3481 gctcactcat taggcaccgg gatctcgacc gatgcccttg agagccttca acccagtcag 3541 ctccttccgg tgggcgcggg gcatgactag catgatcgtg ctcctgtcgt tgaggacccg 3601 gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac 3661 gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg 3721 tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg 3781 gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg tattaacgaa 3841 gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata ccgccagttg 3901 tttaccctca caacgttcca gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag 3961 catcctctct cgtttcatcg gtatcattac ccccatgaac agaaatcccc cttacacgga 4021 ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca 4081 gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg cagacatctg 4141 tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga 4201 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc 4261 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg 4321 cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca 4381 tcagagcaga ttgtactgag agtgcaccat atatgcggtg tgaaataccg cacagatgcg 4441 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct 4501 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 4561 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 4621 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 4681 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 4741 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 4801 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 4861 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 4921 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 4981 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 5041 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 5101 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 5161 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 5221 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 5281 acgaaaactc acgttaaggg attttggtca tgaacaataa aactgtctgc ttacataaac 5341 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcttgctc taggccgcga 5401 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg 5461 caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga gttgtttctg 5521 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg 5581 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca 5641 tggttactca ccactgcgat ccccggcaaa acagcattcc aggtattaga agaatatcct 5701 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt 5761 cctgtttgta attgtccttt taacagtgat cgcgtatttc gtctcgctca ggcgcaatca 5821 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct 5881 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc 5941 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt 6001 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac 6061 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat 6121 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaagaatta 6181 attcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 6241 cacatttccc cgaaaagtgc cacctgaaat tgtaaacgtt aatattttgt taaaattcgc 6301 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc 6361 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag 6421 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga 6481 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc 6541 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa 6601 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt 6661 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc 6721 gtcccattcg cca //
pET-50b(+)载体载体特征
一级属性 | 二级属性 | 属性值 | 起始位置 |
常规 | 长度 | 6733 | |
常规 | 描述 | Bacterial vector for expressing proteins with a cleavable N-terminal NusA tag and a cleavable C-terminal S-tag. | |
常规 | 是否环形 | 1 | |
常规 | 作者 | Novagen (EMD Millipore) | |
常规 | 注释 | ||
来源 | 实验宿主 | Escherichia coli | 1..6733 |
来源 | 分子类型 | other DNA | 1..6733 |
来源 | 种属 | synthetic DNA construct | 1..6733 |
终止子 | 备注 | T7 terminator,transcription terminator for bacteriophage T7 RNA polymerase | 26..73 |
编码区 | 翻译起始位点 | 1 | 168..212 |
编码区 | 备注 | S-Tag | 168..212 |
编码区 | 产物 | affinity and epitope tag derived from pancreatic ribonuclease A | 168..212 |
编码区 | 翻译序列 | KETAAAKFERQHMDS | 168..212 |
编码区 | 翻译起始位点 | 1 | 213..230 |
编码区 | 备注 | thrombin site | 213..230 |
编码区 | 产物 | thrombin recognition and cleavage site | 213..230 |
编码区 | 翻译序列 | LVPRGS | 213..230 |
其它特征区 | 备注 | MCS,multiple cloning site | 234..308 |
编码区 | 翻译起始位点 | 1 | 309..332 |
编码区 | 备注 | HRV 3C site | 309..332 |
编码区 | 产物 | recognition and cleavage site for human rhinovirus 3C protease | 309..332 |
编码区 | 翻译序列 | LEVLFQGP | 309..332 |
编码区 | 翻译起始位点 | 1 | 342..359 |
编码区 | 备注 | 6xHis | 342..359 |
编码区 | 产物 | 6xHis affinity tag | 342..359 |
编码区 | 翻译序列 | HHHHHH | 342..359 |
编码区 | 翻译起始位点 | 1 | 375..1859 |
编码区 | 基因 | nusA | 375..1859 |
编码区 | 备注 | NusA,highly soluble in E. coli | 375..1859 |
编码区 | 产物 | transcription elongation protein (N utilization substance protein A) | 375..1859 |
编码区 | 翻译序列 | MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEIDVRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTFDRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEAVILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGEEVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLWDDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGWELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKELLEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAARGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA | 375..1859 |
编码区 | 翻译起始位点 | 1 | 1866..1883 |
编码区 | 备注 | 6xHis | 1866..1883 |
编码区 | 产物 | 6xHis affinity tag | 1866..1883 |
编码区 | 翻译序列 | HHHHHH | 1866..1883 |
编码区 | 翻译起始位点 | 1 | 1893..1895 |
编码区 | 备注 | ATG | 1893..1895 |
编码区 | 产物 | start codon | 1893..1895 |
编码区 | 翻译序列 | M | 1893..1895 |
核糖体结合位点 | 备注 | ribosome binding site | 1904..1909 |
蛋白结合位点 | 嵌合范围 | lac repressor encoded by lacI | 1940..1964 |
蛋白结合位点 | 备注 | lac operator,The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG). | 1940..1964 |
启动子 | 备注 | T7 promoter,promoter for bacteriophage T7 RNA polymerase | 1965..1983 |
启动子 | 基因 | lacI | 2296..2373 |
启动子 | 备注 | lacI promoter, | 2296..2373 |
编码区 | 翻译起始位点 | 1 | 2374..3456 |
编码区 | 基因 | lacI | 2374..3456 |
编码区 | 备注 | lacI,The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG). | 2374..3456 |
编码区 | 产物 | lac repressor | 2374..3456 |
编码区 | 翻译序列 | MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ | 2374..3456 |
编码区 | 翻译起始位点 | 1 | 4028..4219 |
编码区 | 基因 | rop | 4028..4219 |
编码区 | 备注 | rop | 4028..4219 |
编码区 | 产物 | Rop protein | 4028..4219 |
编码区 | 翻译序列 | MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHADELYRSCLARFGDDGENL | 4028..4219 |
复制起点 | 方向 | LEFT | 4649..5237 |
复制起点 | 备注 | ori,high-copy-number ColE1/pMB1/pBR322/pUC origin of replication | 4649..5237 |
编码区 | 翻译起始位点 | 1 | 5359..6174 |
编码区 | 基因 | aph(3')-Ia | 5359..6174 |
编码区 | 备注 | KanR,confers resistance to kanamycin | 5359..6174 |
编码区 | 产物 | aminoglycoside phosphotransferase | 5359..6174 |
编码区 | 翻译序列 | MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF | 5359..6174 |
复制起点 | 方向 | LEFT | 6267..6722 |
复制起点 | 备注 | f1 ori,f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis | 6267..6722 |
我们不提供该载体,载体信息仅供查询和参考。