- 载体名称:
- pETDuet-1
- 载体抗性:
- Ampicillin
- 载体长度:
- 5420 bp
- 载体类型:
- pET & Duet Vectors (Novagen)
- 复制子:
- ori
- 载体来源:
- Novagen (EMD Millipore)
- 拷贝数:
- High copy number
pETDuet-1 载体载体图谱
pETDuet-1 载体载体序列
LOCUS pETDuet-1. 5420 bp DNA circular SYN 01-JAN-1980 DEFINITION Bacterial vector for the co-expression of two genes. ACCESSION . VERSION . KEYWORDS pETDuet-1. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5420) AUTHORS Novagen (EMD Millipore) TITLE Direct Submission REFERENCE 2 (bases 1 to 5420) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..5420 /mol_type="other DNA" /organism="synthetic DNA construct" protein_bind 3..27 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 42..64 /label=RBS /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 71..73 /codon_start=1 /product="start codon" /label=start codon /note="ATG" /translation="M" CDS 83..100 /label=6xHis /note="6xHis affinity tag" promoter 214..232 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 233..257 /label=lac repressor encoded by lacI binding site /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 286..291 /note="ribosome binding site" CDS 300..302 /codon_start=1 /product="start codon" /label=start codon /note="ATG" /translation="M" CDS 366..410 /label=S-Tag /note="affinity and epitope tag derived from pancreatic ribonuclease A" terminator 462..509 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" rep_origin 546..1001 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" CDS complement(1119..1976) /label=AmpR /note="beta-lactamase" promoter complement(1977..2069) /label=AmpR promoter rep_origin 2150..2738 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" misc_feature complement(2924..3066) /label=bom /note="basis of mobility region from pBR322" CDS complement(3171..3359) /label=rop /note="Rop protein, which maintains plasmids at low copy number" protein_bind complement(3897..3918) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." CDS complement(3934..5013) /label=lacI /note="lac repressor" promoter complement(5014..5091) /label=lacI promoter
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