纽普生物

pPICZα A载体

载体基本信息
载体名称 pPICZα A 载体抗性 Bleomycin
载体长度 3593 bp 载体类型 Yeast Plasmids
载体来源 Invitrogen (Life Technologies) 拷贝数 High copy number

pPICZα A载体质粒图谱

pPICZα A载体质粒图谱

pPICZα A载体载体序列

LOCUS       Exported                3593 bp ds-DNA    circular SYN 05-2-2013
DEFINITION  Pichia pastoris vector for methanol-inducible expression of a 
            secreted protein.
ACCESSION   .
VERSION     .
KEYWORDS    pPICZ(alpha) A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
  JOURNAL   Exported 2017/3/1 from SnapGene 2.3.2
            http://www.snapgene.com
COMMENT     Linearize with SacI, PmeI, or BstXI for integration at the AOX1 
            locus.
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="synthetic DNA construct"
                     /lab_host="Pichia pastoris"
                     /mol_type="other DNA"
     promoter        2..940
                     /gene="Pichia pastoris AOX1"
                     /note="AOX1 promoter"
                     /note="inducible promoter, regulated by methanol"
     CDS             941..1207
                     /codon_start=1
                     /gene="MF-alpha-1"
                     /product="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /note="alpha-factor secretion signal"
                     /note="Cleavage by the Kex2 protease occurs after the 
                     dibasic KR sequence. The EA dipeptides are then removed by 
                     dipeptidyl aminopeptidase A."
                     /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKREAEA"
     misc_feature    1208..1276
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             1275..1304
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /note="Myc"
                     /translation="EQKLISEEDL"
     CDS             1320..1337
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     terminator      1417..1663
                     /gene="Pichia pastoris AOX1"
                     /note="AOX1 terminator"
                     /note="transcription terminator for AOX1"
     promoter        1703..2089
                     /gene="S. cerevisiae TEF1"
                     /note="TEF1 promoter"
                     /note="promoter for EF-1-alpha"
     promoter        2097..2144
                     /note="EM7 promoter"
                     /note="synthetic bacterial promoter "
     CDS             2163..2537
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /note="BleoR"
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      2603..2850
                     /gene="S. cerevisiae CYC1"
                     /note="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     rep_origin      complement(2925..3513)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
      181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
      241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
      301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
      361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
      421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
      481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcggca taccgtttgt
      541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
      601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
      661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
      721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
      781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
      841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
      901 caacttgaga agatcaaaaa acaactaatt attcgaaacg atgagatttc cttcaatttt
      961 tactgctgtt ttattcgcag catcctccgc attagctgct ccagtcaaca ctacaacaga
     1021 agatgaaacg gcacaaattc cggctgaagc tgtcatcggt tactcagatt tagaagggga
     1081 tttcgatgtt gctgttttgc cattttccaa cagcacaaat aacgggttat tgtttataaa
     1141 tactactatt gccagcattg ctgctaaaga agaaggggta tctctcgaga aaagagaggc
     1201 tgaagctgaa ttcacgtggc ccagccggcc gtctcggatc ggtacctcga gccgcggcgg
     1261 ccgccagctt tctagaacaa aaactcatct cagaagagga tctgaatagc gccgtcgacc
     1321 atcatcatca tcatcattga gtttgtagcc ttagacatga ctgttcctca gttcaagttg
     1381 ggcacttacg agaagaccgg tcttgctaga ttctaatcaa gaggatgtca gaatgccatt
     1441 tgcctgagag atgcaggctt catttttgat acttttttat ttgtaaccta tatagtatag
     1501 gatttttttt gtcattttgt ttcttctcgt acgagcttgc tcctgatcag cctatctcgc
     1561 agctgatgaa tatcttgtgg taggggtttg ggaaaatcat tcgagtttga tgtttttctt
     1621 ggtatttccc actcctcttc agagtacaga agattaagtg agaccttcgt ttgtgcggat
     1681 cccccacaca ccatagcttc aaaatgtttc tactcctttt ttactcttcc agattttctc
     1741 ggactccgcg catcgccgta ccacttcaaa acacccaagc acagcatact aaattttccc
     1801 tctttcttcc tctagggtgt cgttaattac ccgtactaaa ggtttggaaa agaaaaaaga
     1861 gaccgcctcg tttctttttc ttcgtcgaaa aaggcaataa aaatttttat cacgtttctt
     1921 tttcttgaaa tttttttttt tagttttttt ctctttcagt gacctccatt gatatttaag
     1981 ttaataaacg gtcttcaatt tctcaagttt cagtttcatt tttcttgttc tattacaact
     2041 ttttttactt cttgttcatt agaaagaaag catagcaatc taatctaagg ggcggtgttg
     2101 acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa
     2161 ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg
     2221 tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg
     2281 gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg
     2341 acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg
     2401 aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc
     2461 agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg
     2521 ccgaggagca ggactgacac gtccgacggc ggcccacggg tcccaggcct cggagatccg
     2581 tccccctttt cctttgtcga tatcatgtaa ttagttatgt cacgcttaca ttcacgccct
     2641 ccccccacat ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct
     2701 atttattttt ttatagttat gttagtatta agaacgttat ttatatttca aatttttctt
     2761 ttttttctgt acagacgcgt gtacgcatgt aacattatac tgaaaacctt gcttgagaag
     2821 gttttgggac gctcgaaggc tttaatttgc aagctggaga ccaacatgtg agcaaaaggc
     2881 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     2941 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     3001 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     3061 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa
     3121 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     3181 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     3241 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     3301 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     3361 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     3421 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     3481 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     3541 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag atc
//

pPICZα A载体载体特征

一级属性 二级属性 属性值 起始位置
常规长度3593
常规描述Pichia pastoris vector for methanol-inducible expression of a secreted protein.
常规是否环形1
常规作者Invitrogen (Life Technologies)
常规注释
来源实验宿主Pichia pastoris1..3593
来源分子类型other DNA1..3593
来源种属synthetic DNA construct1..3593
启动子基因Pichia pastoris AOX12..940
启动子备注AOX1 promoter,inducible promoter, regulated by methanol2..940
编码区翻译起始位点1941..1207
编码区基因MF-alpha-1941..1207
编码区备注alpha-factor secretion signal,Cleavage by the Kex2 protease occurs after the dibasic KR sequence. The EA dipeptides are then removed by dipeptidyl aminopeptidase A.941..1207
编码区产物N-terminal secretion signal from S. cerevisiae alpha-factor941..1207
编码区翻译序列MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKREAEA941..1207
其它特征区备注MCS,multiple cloning site1208..1276
编码区翻译起始位点11275..1304
编码区备注Myc1275..1304
编码区产物Myc (human c-Myc oncogene) epitope tag1275..1304
编码区翻译序列EQKLISEEDL1275..1304
编码区翻译起始位点11320..1337
编码区备注6xHis1320..1337
编码区产物6xHis affinity tag1320..1337
编码区翻译序列HHHHHH1320..1337
终止子基因Pichia pastoris AOX11417..1663
终止子备注AOX1 terminator,transcription terminator for AOX11417..1663
启动子基因S. cerevisiae TEF11703..2089
启动子备注TEF1 promoter,promoter for EF-1-alpha1703..2089
启动子备注EM7 promoter,synthetic bacterial promoter 2097..2144
编码区翻译起始位点12163..2537
编码区基因Sh ble from Streptoalloteichus hindustanus2163..2537
编码区备注BleoR,confers resistance to bleomycin, phleomycin, and Zeocin(TM)2163..2537
编码区产物antibiotic-binding protein2163..2537
编码区翻译序列MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGREFALRDPAGNCVHFVAEEQD2163..2537
终止子基因S. cerevisiae CYC12603..2850
终止子备注CYC1 terminator,transcription terminator for CYC12603..2850
复制起点方向LEFT2925..3513
复制起点备注ori,high-copy-number ColE1/pMB1/pBR322/pUC origin of replication2925..3513

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