- 蛋白种属:人
- 表达宿主:人胚胎肾细胞(HEK293)
- Unitprot ID:P04629
产品详情
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产品名称
重组人NTRK1蛋白
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蛋白种属
人
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表达宿主
人胚胎肾细胞(HEK293)
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Unitprot ID
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克隆构建
A DNA sequence encoding the human NTRK1 (NP_002520.2) extracellular domain (Met 1-Pro 382) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
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蛋白纯度
> 98 % as determined by SDS-PAGE
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蛋白活性
Measured by its ability to inhibit NGF-induced proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.04-0.15 μg/ml in the presence of 10 ng/ml of recombinant human NGF.
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分子量
The recombinant human NTRK1/Fc is a disulfide-linked homodimer. The reduced monomer consists of 598 amino acids and has a predicted molecular mass of 66 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh NTRK1/Fc monomer is approximately 85-95 kDa due to glycosylation.
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蛋白别名
TRK-A; TRKA; NTRK1; C80751; P140-TRKA; DKFZP781I14186; MTC; TKR; TRK; TRK1
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蛋白全名
High affinity nerve growth factor receptor
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蛋白描述
TRKA is a member of the neurotrophic tyrosine kinase receptor (NTKR) family. It is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed. Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors. The presence of NTRK1 leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in TRKA gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. It was originally identified as an oncogene as it is commonly mutated in cancers, particularly colon and thyroid carcinomas. TRKA is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). Known substrates for the Trk receptors are SHC1, PI 3-kinase, and PLC-gamma-1. NTRK1 has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. It also activates ERK1 by either SHC1- or PLC-gamma-1-dependent signaling pathway. Defects in NTRK1 are a cause of congenital insensitivity to pain with anhidrosis and thyroid papillary carcinoma.
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发货形式
Lyophilized from sterile PBS, pH 7.4
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.
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内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
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保存条件
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
重组蛋白定制服务:
纽普生物(NovoPro)提供大肠杆菌、哺乳细胞、昆虫细胞和酵母细胞重组蛋白定制服务
声明:本公司出售产品只能用于科研目的,不得用于诊断或者治疗!