重组斑马鱼EFNB2A蛋白

产品详情

  • 产品名称
    重组斑马鱼EFNB2A蛋白
  • 蛋白种属
    斑马鱼
  • 表达宿主
    人胚胎肾细胞（HEK293）
  • Unitprot ID
    O73874
  • 克隆构建
    A DNA sequence encoding the zebrafish EFNB2A (O73874) (Met 1-Ala222) was expressed with the Fc region of human IgG1 at the C-terminus.
  • 蛋白纯度
    (16.2+75.4) % as determined by SDS-PAGE
  • 蛋白活性
    Measured by its binding ability in a functional ELISA.
    Immobilized human EphB4-His (Cat:502026) at 10 μg/ml (100 μl/well) can bind zebrafish EFNB2A-Fc, The EC50 of zebrafish EFNB2A-Fc is 12.8-30.1 ng/ml.
  • 分子量
    The recombinant zebrafish EFNB2A consists of 439 amino acids and has a calculated molecular mass of 48.9 kDa. It migrates as an 97 and 58 KDa band in SDS-PAGE under reducing conditions.
  • 蛋白别名
    ID:IBD5072; EFNB2; CH211-149B20.1
  • 蛋白全名
    Ephrin-B2a
  • 蛋白描述
    Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with ephb4 may play a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration (By similarity).
  • 发货形式
    Lyophilized from sterile PBS, PH 7.4.
    1. 5 % trehalose and mannitol are added as protectants before lyophilization.
    2. Please contact us for any concerns or special requirements.
  • 内毒素
    < 1.0 EU per μg of the protein as determined by the LAL method
  • 保存条件
    Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
声明：本公司出售产品只能用于科研目的，不得用于诊断或者治疗！