LOCUS V027096 5683 bp DNA circular SYN 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION V027096
VERSION V027096
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
enhancer 27..330
/note="human cytomegalovirus immediate early enhancer"
/label="CMV enhancer"
promoter 331..534
/note="human cytomegalovirus (CMV) immediate early
promoter"
/label="CMV promoter"
intron 672..768
/note="modified SV40 intron with splice donor and acceptor
sites"
/label="SV40 intron"
CDS 870..2735
/label="COLGALT1(NM_024656)"
/note="COLGALT1(NM_024656)"
/gene="COLGALT1"
CDS 2742..2807
/codon_start=1
/product="three tandem FLAG® epitope tags, followed by an
enterokinase cleavage site"
/transl_table=1
/translation="DYKDHDGDYKDHDIDYKDDDDK"
/label="3xFLAG"
polyA_signal 2822..2956
/note="SV40 polyadenylation signal"
/label="SV40 poly(A) signal"
primer_bind complement(3067..3083)
/note="common sequencing primer, one of multiple similar
variants"
/label="M13 rev"
protein_bind 3091..3107
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG)."
/label="lac operator"
promoter complement(3115..3145)
/note="promoter for the E. coli lac operon"
/label="lac promoter"
protein_bind 3160..3181
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
/label="CAP binding site"
rep_origin complement(3469..4057)
/direction=LEFT
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
/label="ori"
CDS complement(4228..5088)
/codon_start=1
/gene="bla"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/product="β-lactamase"
/transl_table=1
/translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
SLIKHW*"
/label="AmpR"
promoter complement(5089..5193)
/gene="bla"
/label="AmpR promoter"
primer_bind 5667..5683
/note="common sequencing primer, one of multiple similar
variants"
/label="M13 fwd"