LOCUS       V027096                 5683 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027096
VERSION     V027096
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        27..330
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        331..534
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          672..768
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
     CDS             870..2735
                     /label="COLGALT1(NM_024656)"
                     /note="COLGALT1(NM_024656)"
                     /gene="COLGALT1"
     CDS             2742..2807
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    2822..2956
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(3067..3083)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    3091..3107
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(3115..3145)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    3160..3181
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(3469..4057)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(4228..5088)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(5089..5193)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     5667..5683
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaggcc cgaattcggt
      841 cgaccgagat ctctcgaggt accgccacca tggcggcggc cccacgcgcg ggccggcggc
      901 gcgggcagcc gctcctggcg ctgctgcttc tgctgctggc gccactgccg ccgggggccc
      961 cgccgggcgc cgacgcctac ttccccgagg agcgctggag cccggagtcg cccctgcagg
     1021 cgccgcgcgt gctcatcgcg ctgttggcgc gaaacgcggc ccacgcgttg cccaccacgc
     1081 tgggcgcact cgagcggctg cggcacccgc gggagcgcac ggcgctatgg gtggctacgg
     1141 accacaacat ggataacacg tcaactgtgc tgcgggagtg gctggtggcc gtgaagagtt
     1201 tgtaccattc cgtggagtgg cggccagcag aggagcccag gtcctacccg gacgaggaag
     1261 gcccgaaaca ctggtctgac tcacgctacg agcatgtcat gaagttgcgc caggcagccc
     1321 tgaaatcagc tcgagacatg tgggctgatt acatcctgtt tgtagatgcg gacaacctga
     1381 tcctcaaccc tgacacactg agcctgctca tcgctgagaa caagacggtg gtcgccccca
     1441 tgctggattc ccgggctgcg tactccaact tctggtgtgg aatgacttcc cagggctact
     1501 acaagcgcac acctgcctac atccctatcc gcaagcgaga ccgccggggc tgctttgcag
     1561 ttcccatggt gcactcgacc ttcctgatcg acctgcggaa ggcggcgtcc aggaacctgg
     1621 ccttctaccc acctcaccct gactacacct ggtcctttga cgacatcatc gtctttgcct
     1681 tctcctgcaa gcaggcagag gttcagatgt atgtgtgcaa caaggaggag tacggattct
     1741 tgccagtgcc attgcgcgcc cacagcaccc tccaggatga ggccgagagc ttcatgcatg
     1801 tgcagctgga ggtcatggtg aagcacccgc ccgcagagcc ctcccgcttc atctcggctc
     1861 ccaccaagac accggacaag atgggcttcg acgaggtctt catgatcaac ctgaggcggc
     1921 ggcaggaccg gcgggagcgc atgctgcggg cgctgcaggc acaggagatc gagtgccggc
     1981 tggtggaggc cgtggacggc aaagccatga acaccagcca ggtggaggcg ctggggatcc
     2041 agatgctgcc tggctaccgg gacccctacc acggccggcc cctcaccaag ggtgagctgg
     2101 gctgcttcct gagccactac aacatctgga aggaggtggt ggaccggggg ctgcagaaat
     2161 cgcttgtgtt tgaggatgac ctgcgttttg agatcttctt caagagacgt ctgatgaacc
     2221 tcatgcggga tgtggagcgg gagggcctgg actgggacct catctatgtg ggccggaagc
     2281 ggatgcaggt ggagcacccc gagaaggctg tgcctcgcgt gaggaacctg gtggaggccg
     2341 actattccta ctggaccctg gcctacgtga tctccctgca aggcgcccgc aaactgctgg
     2401 ctgctgagcc gctctccaag atgctgcctg tggacgagtt cctgcccgtc atgttcgaca
     2461 aacacccagt gtccgagtac aaggcccact tctccctccg caacctgcat gccttctctg
     2521 tggagccgct gctcatctac cccacacact acacaggaga cgatggctat gtgagtgaca
     2581 ccgagacctc agtcgtatgg aacaatgagc acgtcaagac cgactgggac cgcgccaagt
     2641 cccagaagat gcgggagcag caggcactga gccgtgaggc caagaactcg gacgtgctcc
     2701 agtccccact ggacagtgct gcccgggatg aactcggatc cgactacaaa gaccatgacg
     2761 gtgattataa agatcatgac atcgactaca aggacgacga tgacaagtaa gcggccgcgg
     2821 ggatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     2881 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     2941 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     3001 ggtgtgggag gttttttcgg atcctctaga gtcgatctgc aggcatgcta gcttggcgta
     3061 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     3121 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     3181 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     3241 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     3301 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     3361 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     3421 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     3481 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     3541 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     3601 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     3661 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     3721 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     3781 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     3841 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     3901 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     3961 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     4021 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     4081 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     4141 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     4201 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     4261 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     4321 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     4381 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     4441 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     4501 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     4561 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     4621 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     4681 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     4741 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     4801 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     4861 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     4921 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     4981 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     5041 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     5101 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     5161 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     5221 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     5281 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     5341 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     5401 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
     5461 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     5521 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     5581 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     5641 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agt
//
