我们所展示的质粒图谱主要是从文献和开放数据库中收集而来，主要是为了方便研究工作，其中一小部分质粒进行了质量控制，可供科学家使用。
所有的产品都严格仅供学术研究使用，不能应用于临床试验，包括人体摄入、注射或外用的药理学用途。
确保质粒的关键元件正确，但是我们并不能保证实验结果。页面展示的图谱序列为理论序列，可能与测序结果不一致，请自行比对后确定是否满足要求。(如果测过序，本页面一般会提供下载)
开放数据库中的大多数载体序列都没有被完全测序。如果实际序列与参考序列的相似度超过99%，则将其视为正确。
由于科学研究是在探索未知，具有很大的不确定性，在任何情况下，我们都不承担超出质粒本身的额外经济损失或责任。
Note:
- 载体名称:
- pSB1C3_I0500_LuxI
- 载体抗性:
- Chloramphenicol
- 载体长度:
- 3891 bp
- 载体类型:
- Cloning vector
- 复制子:
- ori
- 载体来源:
- Boehm CR, Grant PK, Haseloff J.
- 启动子:
- araBAD
pSB1C3_I0500_LuxI 载体图谱
质粒操作方法
1. 发货形式：质粒干粉(常温运输，存于-20度，请务必先转化提质粒后使用)
2. 收到质粒干粉后请先5000rpm离心1min，再加入20μl ddH2O溶解质粒;（质粒复测的浓度有时候与标称值差距较大，这可能是因为冻干质粒在管中的位置、复溶效率、测量偏差以及管壁的吸附导致，因此建议先转化提质粒后再使用）
3. 取1支100μl 感受态于冰上解冻10min，加入2μl质粒，再冰浴30min后，42℃热激60s，不要搅动，再冰浴2min；
4. 加入900μl无抗的LB液体培养基，180rpm震荡37℃培养45min (30℃培养1-1.5小时);
5. 6000rpm离心5min，仅留100μl上清液重悬细菌沉淀，并涂布至目标质粒抗性的LB平板上;
6. 将平板倒置37℃培养14h，如果要求是30℃则培养20h; (菌落过多则将质粒稀释后再转化；没有菌落则加入10μl质粒转化；建议不要直接转表达感受态， 要先转克隆感受态，重提质粒后再导入表达感受态);
7. 挑取单菌落至LB液体培养基中，加入对应抗生素，220rpm震荡培养14h，根据实验需要和质粒提取试剂盒说明书提取质粒。
pSB1C3_I0500_LuxI 载体序列
LOCUS V003461 3891 bp DNA circular SYN 18-DEC-2018
DEFINITION Exported.
ACCESSION V003461
VERSION V003461
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 3891)
AUTHORS Boehm CR, Grant PK, Haseloff J.
TITLE Programmed emergence of a domain of gene expression in a bacterial
population
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 3891)
AUTHORS Boehm CR.
TITLE Direct Submission
JOURNAL Submitted (12-OCT-2016) Plant Sciences, University of Cambridge,
Downing Street, Cambridge, Cambridgeshire CB2 3EA, United Kingdom
REFERENCE 3 (bases 1 to 3891)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 3891)
AUTHORS .
TITLE Direct Submission
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(12-OCT-2016) Plant Sciences, University of Cambridge, Downing
Street, Cambridge, Cambridgeshire CB2 3EA, United Kingdom"
SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..3891
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(4..879)
/label="araC"
/note="L-arabinose regulatory protein"
promoter 906..1190
/label="araBAD promoter"
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
regulatory 1219..1230
/label="BBa_B0034"
/note="BBa_B0034"
/regulatory_class="ribosome_binding_site"
RBS 1219..1230
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 1237..1815
/gene="luxI"
/label="Acyl-homoserine-lactone synthase"
/note="Acyl-homoserine-lactone synthase from Aliivibrio
fischeri. Accession#: P12747"
misc_feature 1822..1842
/label="BioBrick suffix"
/note="universal suffix for all parts"
terminator 1843..1900
/label="his operon terminator"
/note="This putative transcriptin terminator from the E.
coli his operon has a 2-bp deletion introduced during
synthesis. Its efficiency has not been determined."
rep_origin complement(2095..2683)
/direction=LEFT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
terminator complement(2866..2960)
/label="lambda t0 terminator"
/note="transcription terminator from phage lambda"
CDS complement(2984..3640)
/label="CmR"
/note="chloramphenicol acetyltransferase"
promoter complement(3641..3744)
/label="cat promoter"
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
terminator complement(3824..3867)
/label="bacterial terminator"
/note="putative bacterial transcription terminator"
misc_feature 3870..3891
/label="BioBrick prefix"
/note="BioBrick prefix for parts that do not start with
'ATG'"