LOCUS       V003461                 3891 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003461
VERSION     V003461
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Boehm CR, Grant PK, Haseloff J.
  TITLE     Programmed emergence of a domain of gene expression in a bacterial
            population
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Boehm CR.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2016) Plant Sciences, University of Cambridge,
            Downing Street, Cambridge, Cambridgeshire CB2 3EA, United Kingdom
REFERENCE   3  (bases 1 to 3891)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3891)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-OCT-2016) Plant Sciences, University of Cambridge, Downing
            Street, Cambridge, Cambridgeshire CB2 3EA, United Kingdom"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(4..879)
                     /label="araC"
                     /note="L-arabinose regulatory protein"
     promoter        906..1190
                     /label="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite
                     direction (Guzman et al., 1995)"
     regulatory      1219..1230
                     /label="BBa_B0034"
                     /note="BBa_B0034"
                     /regulatory_class="ribosome_binding_site"
     RBS             1219..1230
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     CDS             1237..1815
                     /gene="luxI"
                     /label="Acyl-homoserine-lactone synthase"
                     /note="Acyl-homoserine-lactone synthase from Aliivibrio
                     fischeri. Accession#: P12747"
     misc_feature    1822..1842
                     /label="BioBrick suffix"
                     /note="universal suffix for all parts"
     terminator      1843..1900
                     /label="his operon terminator"
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during
                     synthesis. Its efficiency has not been determined."
     rep_origin      complement(2095..2683)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     terminator      complement(2866..2960)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(2984..3640)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3641..3744)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     terminator      complement(3824..3867)
                     /label="bacterial terminator"
                     /note="putative bacterial transcription terminator"
     misc_feature    3870..3891
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatga ctataatgat aaaaaaatcg
     1261 gattttttgg caattccatc ggaggagtat aaaggtattc taagtcttcg ttatcaagtg
     1321 tttaagcaaa gacttgagtg ggacttagtt gtagaaaata accttgaatc agatgagtat
     1381 gataactcaa atgcagaata tatttatgct tgtgatgata ctgaaaatgt aagtggatgc
     1441 tggcgtttat tacctacaac aggtgattat atgctgaaaa gtgtttttcc tgaattgctt
     1501 ggtcaacaga gtgctcccaa agatcctaat atagtcgaat taagtcgttt tgctgtaggt
     1561 aaaaatagct caaagataaa taactctgct agtgaaatta caatgaaact atttgaagct
     1621 atatataaac acgctgttag tcaaggtatt acagaatatg taacagtaac atcaacagca
     1681 atagagcgat ttttaaagcg tattaaagtt ccttgtcatc gtattggaga caaagaaatt
     1741 catgtattag gtgatactaa atcggttgta ttgtctatgc ctattaatga acagtttaaa
     1801 aaagcagtct taaattaata atactagtag cggccgctgc agtccggcaa aaaagggcaa
     1861 ggtgtcacca ccctgccctt tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc
     1921 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     1981 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     2041 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     2101 caggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     2161 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     2221 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     2281 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     2341 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     2401 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     2461 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     2521 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     2581 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     2641 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     2701 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     2761 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     2821 ctaaagtata tatgagtaaa cttggtctga cagctcgagg cttggattct caccaataaa
     2881 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
     2941 tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact
     3001 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
     3061 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
     3121 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
     3181 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
     3241 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     3301 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     3361 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
     3421 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
     3481 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
     3541 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     3601 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
     3661 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
     3721 tggaacctct tacgtgcccg atcaactcga gtgccacctg acgtctaaga aaccattatt
     3781 atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa
     3841 tccttagctt tcgctaagga tgatttctgg aattcgcggc cgcttctaga g
//
