- 载体名称:
- pLac-EGFP-Tac-SUL1-Tet-tetG
- 载体抗性:
- Kanamycin
- 载体长度:
- 6201 bp
- 载体类型:
- Protein expression
- 复制子:
- ori
- 宿主:
- E. coli
- 启动子:
- tet
pLac-EGFP-Tac-SUL1-Tet-tetG 载体载体图谱
pLac-EGFP-Tac-SUL1-Tet-tetG 载体载体序列
LOCUS 62056_13670 6201 bp DNA circular SYN 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6201)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..6201
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 33..54
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 69..99
/label=lac promoter
/note="promoter for the E. coli lac operon"
CDS 138..854
/label=EGFP
/note="enhanced GFP"
promoter 871..899
/label=tac promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
CDS 929..1765
/gene="sulI"
/label=sulI
/note="Dihydropteroate synthase type-1 from Escherichia
coli. Accession#: P0C002"
promoter 1785..1813
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
polyA_signal 2989..3110
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(3117..3572)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3599..3703
/label=AmpR promoter
promoter 3705..4062
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 4097..4888
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
polyA_signal 5123..5170
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5499..6087
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
我们不提供该载体，载体信息仅供查询和参考。