LOCUS       62056_13670        6201 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6201)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    33..54
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        69..99
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     CDS             138..854
                     /label=EGFP
                     /note="enhanced GFP"
     promoter        871..899
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     CDS             929..1765
                     /gene="sulI"
                     /label=sulI
                     /note="Dihydropteroate synthase type-1 from Escherichia
                     coli. Accession#: P0C002"
     promoter        1785..1813
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     polyA_signal    2989..3110
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3117..3572)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3599..3703
                     /label=AmpR promoter
     promoter        3705..4062
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4097..4888
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5123..5170
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5499..6087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattac tagcgctacc ggactcagat cttaatgtga gttagctcac tcattaggca
       61 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaatcta gcggatactt
      121 tcacacagga aacagctatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
      181 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
      241 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
      301 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
      361 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
      421 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
      481 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
      541 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
      601 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
      661 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
      721 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
      781 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
      841 acgagctgta caagtaaagc ggccgagctg ttgacaatta atcatcggct cgtataatgt
      901 gtggaatttc acacaggaaa cagtattcat ggtgacggtg ttcggcattc tgaatctcac
      961 cgaggactcc ttcttcgatg agagccggcg gctagacccc gccggcgctg tcaccgcggc
     1021 gatcgaaatg ctgcgagtcg gatcagacgt cgtggatgtc ggaccggccg ccagccatcc
     1081 ggacgcgagg cctgtatcgc cggccgatga gatcagacgt attgcgccgc tcttagacgc
     1141 cctgtccgat cagatgcacc gtgtttcaat cgacagcttc caaccggaaa cccagcgcta
     1201 tgcgctcaag cgcggcgtgg gctacctgaa cgatatccaa ggatttcctg accctgcgct
     1261 ctatcccgat attgctgagg cggactgcag gctggtggtt atgcactcag cgcagcggga
     1321 tggcatcgcc acccgcaccg gtcaccttcg acccgaagac gcgctcgacg agattgtgcg
     1381 gttcttcgag gcgcgggttt ccgccttgcg acggagcggg gtcgctgccg accggctcat
     1441 cctcgatccg gggatgggat ttttcttgag ccccgcaccg gaaacatcgc tgcacgtgct
     1501 gtcgaacctt caaaagctga agtcggcgtt ggggcttccg ctattggtct cggtgtcgcg
     1561 gaaatccttc ttgggcgcca ccgttggcct tcctgtaaag gatctgggtc cagcgagcct
     1621 tgcggcggaa cttcacgcga tcggcaatgg cgctgactac gtccgcaccc acgcgcctgg
     1681 agatctgcga agcgcaatca ccttctcgga aaccctcgcg aaatttcgca gtcgcgacgc
     1741 cagagaccga gggttagatc atgcctgaaa gcgacaggcc atgtttgaca gcttatcatc
     1801 gataagcttt aatgcggtag tttatcacag ttaaattgct aacgcaatgg gtctcggcct
     1861 catcatgccc gtccttccga cgcttctgcg tgagcttgtg ccagcagagc aggtcgctgg
     1921 acactatggt gccttgctgt cgctctatgc attgatgcag gtcgtcttcg cgcccatgct
     1981 tggacagctt tcggattctt acggtcggcg tccggtactt ctggcttctc ttgcaggagc
     2041 cgcagtcgat tacacgatta tggcatcagc gccggtctta tgggtgctct atatcggccg
     2101 actcgtgtcc ggcgtcacgg gcgcaaccgg agctgtagca gcctcaacca ttgccgattc
     2161 gacgggggaa ggttctcgcg cacgctggtt cggctacatg ggggcctgtt atggggcggg
     2221 catgattgcc gggccagcac ttggtggcat gctcggtggt atctctgctc atgccccgtt
     2281 tatcgccgcc gcccttctca acgggttcgc gttcctgctt gcctgcattt tcctcaagga
     2341 gactcatcac agccatggcg ggaccggaaa gccggttcgc atcaaaccat tcgttctgtt
     2401 acggctggat gatgcattgc gcgggctagg tgcgcttttc gcagttttct tcattattca
     2461 actgatcggc caagtgcctg cagccctatg ggtcatatat ggcgaggacc gttttcagtg
     2521 gaacaccgcg accgttggtt tgtcgctcgc ggcgtttggg gcaacacatg cgatcttcca
     2581 agcgtttgtt accggcccgc tttcaagccg gcttggagag cggcgcacgc tgctgtttgg
     2641 catggctgcg gatgcgactg gcttcgttct tctggctttt gccacgcagg gatggatggt
     2701 gttcccgatt ctgttgctgc ttgccgccgg gggtgttggc atgccggcct tgcaggcaat
     2761 gctctcaaac aatgtcagca gtaacaagca aggggctttg caaggaacgc taacgagcct
     2821 caccaatcta agctctatcg caggaccgct tggcttcaca gcactctatt ctgccaccgc
     2881 cggggcatgg aacggttggg tttggattgt cggcgcgatc ctctatttaa tatgtctgcc
     2941 aatactacgc agaccattcg caacttcatt gtgacaattg ttgttgttaa cttgtttatt
     3001 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     3061 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat
     3121 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     3181 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     3241 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     3301 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     3361 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     3421 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     3481 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     3541 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg
     3601 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     3661 ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac
     3721 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     3781 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     3841 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     3901 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     3961 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     4021 aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg
     4081 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     4141 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     4201 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     4261 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     4321 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     4381 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     4441 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     4501 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     4561 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     4621 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     4681 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     4741 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     4801 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     4861 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     4921 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     4981 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     5041 atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata
     5101 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg
     5161 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
     5221 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
     5281 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca
     5341 ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     5401 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     5461 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     5521 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     5581 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     5641 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     5701 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     5761 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     5821 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     5881 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     5941 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     6001 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6061 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     6121 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     6181 aaccgtatta ccgccatgca t
//
