LOCUS V041273 10909 bp DNA circular SYN 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION V041273
VERSION V041273
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
LTR 1..634
/note="3' long terminal repeat (LTR) from HIV-1"
/label="3' LTR"
misc_feature 681..806
/note="packaging signal of human immunodeficiency virus
type 1"
/label="HIV-1 Ψ"
misc_feature 1303..1536
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
/label="RRE"
CDS 1721..1765
/codon_start=1
/note="recognized by the 2H10 single-chain llama nanobody"
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/transl_table=1
/translation="KNEQELLELDKWASL"
/label="gp41 peptide"
misc_feature 2028..2143
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
/label="cPPT/CTS"
enhancer 2201..2504
/note="human cytomegalovirus immediate early enhancer"
/label="CMV enhancer"
promoter 2505..2708
/note="human cytomegalovirus (CMV) immediate early
promoter"
/label="CMV promoter"
CDS 2843..5641
/label="PGR(NM_000926)"
/note="PGR(NM_000926)"
/gene="PGR"
CDS 5642..5665
/codon_start=1
/product="FLAG® epitope tag, followed by an enterokinase
cleavage site"
/transl_table=1
/translation="DYKDDDDK"
/label="FLAG"
promoter 5697..6196
/note="mouse phosphoglycerate kinase 1 promoter"
/label="PGK promoter"
CDS 6217..6816
/codon_start=1
/gene="pac from Streptomyces alboniger"
/note="confers resistance to puromycin"
/product="puromycin N-acetyltransferase"
/transl_table=1
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
/label="PuroR"
misc_feature 6830..7418
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
/label="WPRE"
LTR 7625..8258
/note="3' long terminal repeat (LTR) from HIV-1"
/label="3' LTR"
primer_bind complement(8387..8403)
/note="common sequencing primer, one of multiple similar
variants"
/label="M13 rev"
protein_bind 8411..8427
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG)."
/label="lac operator"
promoter complement(8435..8465)
/note="promoter for the E. coli lac operon"
/label="lac promoter"
protein_bind 8480..8501
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
/label="CAP binding site"
rep_origin complement(8789..9377)
/direction=LEFT
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
/label="ori"
CDS complement(9548..10408)
/codon_start=1
/gene="bla"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/product="β-lactamase"
/transl_table=1
/translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
SLIKHW*"
/label="AmpR"
promoter complement(10409..10513)
/gene="bla"
/label="AmpR promoter"
polyA_signal 10561..10695
/note="SV40 polyadenylation signal"
/label="SV40 poly(A) signal"