我们所展示的质粒图谱主要是从文献和开放数据库中收集而来，主要是为了方便研究工作，其中一小部分质粒进行了质量控制，可供科学家使用。
所有的产品都严格仅供科学研究使用，不能应用于临床试验，包括人体摄入、注射或外用的药理学用途。
确保质粒的关键元件正确，但是我们并不能保证实验结果。页面展示的图谱序列为理论序列，可能与测序结果不一致，请自行比对后确定是否满足要求。(如果测过序，本页面一般会提供下载)
开放数据库中的大多数载体序列都没有被完全测序。如果实际序列与参考序列的相似度超过99%，则将其视为正确。
由于科学研究是在探索未知，具有很大的不确定性，在任何情况下，我们都不承担超出质粒本身的额外经济损失或责任。
- 载体名称:
- pQTEV
- 载体抗性:
- Ampicillin
- 载体长度:
- 4803 bp
- 载体类型:
- Cloning vector
- 复制子:
- ori
- 载体来源:
- Scheich C, Niesen FH, Seckler R, Bussow K.
pQTEV 载体图谱
质粒操作方法
1. 发货形式：质粒干粉(常温运输，存于-20度，请务必先转化提质粒后使用)
2. 收到质粒干粉后请先5000rpm离心1min，再加入20μl ddH2O溶解质粒;（质粒复测的浓度有时候与标称值差距较大，这可能是因为冻干质粒在管中的位置、复溶效率、测量偏差以及管壁的吸附导致，因此建议先转化提质粒后再使用）
3. 取1支100μl 感受态于冰上解冻10min，加入2μl质粒，再冰浴30min后，42℃热激60s，不要搅动，再冰浴2min；
4. 加入900μl无抗的LB液体培养基，180rpm震荡37℃培养45min (30℃培养1-1.5小时);
5. 6000rpm离心5min，仅留100μl上清液重悬细菌沉淀，并涂布至目标质粒抗性的LB平板上;
6. 将平板倒置37℃培养14h，如果要求是30℃则培养20h; (菌落过多则将质粒稀释后再转化；没有菌落则加入10μl质粒转化；建议不要直接转表达感受态， 要先转克隆感受态，重提质粒后再导入表达感受态);
7. 挑取单菌落至LB液体培养基中，加入对应抗生素，220rpm震荡培养14h，根据实验需要和质粒提取试剂盒说明书提取质粒。
pQTEV 载体序列
LOCUS 40924_36258 4803 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pQTEV, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4803)
AUTHORS Scheich C, Niesen FH, Seckler R, Bussow K.
TITLE An automated in vitro protein folding screen applied to a human
dynactin subunit
JOURNAL Protein Sci. 13 (2), 370-380 (2004)
PUBMED 14739323
REFERENCE 2 (bases 1 to 4803)
AUTHORS Bussow K.
TITLE Construction of expression vector pQTEV
JOURNAL Unpublished
REFERENCE 3 (bases 1 to 4803)
AUTHORS Bussow K.
TITLE Direct Submission
JOURNAL Submitted (25-FEB-2003) Prof. H. Lehrach, Max Planck Institute for
Molecular Genetics, Ihnestr. 73, Berlin 14195, Germany
REFERENCE 4 (bases 1 to 4803)
TITLE Direct Submission
REFERENCE 5 (bases 1 to 4803)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Protein
Sci."; date: "2004"; volume: "13"; issue: "2"; pages: "370-380"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
(25-FEB-2003) Prof. H. Lehrach, Max Planck Institute for Molecular
Genetics, Ihnestr. 73, Berlin 14195, Germany"
COMMENT SGRef: number: 4; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..4803
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 10..54
/label=T5 promoter
/note="bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator"
protein_bind 62..78
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 97..108
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
regulatory 101..107
/regulatory_class="ribosome_binding_site"
misc_feature 115
/note="translation start site for inserts cloned into the
multiple cloning site"
CDS 121..141
/label=7xHis
/note="6xHis affinity tag"
CDS 166..186
/label=TEV site
/note="tobacco etch virus (TEV) protease recognition and
cleavage site"
misc_feature 183..244
/note="multiple cloning site; includes BamHI, SalI, BglII,
KpnI, NotI and HindIII restriction sites"
terminator 260..354
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
CDS 398..1054
/label=CmR
/note="chloramphenicol acetyltransferase"
terminator 1122..1208
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
protein_bind complement(1267..1288)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
CDS complement(1304..2383)
/label=lacI
/note="lac repressor"
promoter complement(2384..2461)
/label=lacIq promoter
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
misc_feature 2653..2793
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(2979..3567)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3741..4598)
/label=AmpR
/note="beta-lactamase"
promoter complement(4599..4703)
/label=AmpR promoter