我们所展示的质粒图谱主要是从文献和开放数据库中收集而来，主要是为了方便研究工作，其中一小部分质粒进行了质量控制，可供科学家使用。
所有的产品都严格仅供科学研究使用，不能应用于临床试验，包括人体摄入、注射或外用的药理学用途。
确保质粒的关键元件正确，但是我们并不能保证实验结果。页面展示的图谱序列为理论序列，可能与测序结果不一致，请自行比对后确定是否满足要求。(如果测过序，本页面一般会提供下载)
开放数据库中的大多数载体序列都没有被完全测序。如果实际序列与参考序列的相似度超过99%，则将其视为正确。
由于科学研究是在探索未知，具有很大的不确定性，在任何情况下，我们都不承担超出质粒本身的额外经济损失或责任。
- 载体名称:
- pQLinkHD
- 载体抗性:
- Ampicillin
- 载体长度:
- 7062 bp
- 载体类型:
- Cloning vector
- 复制子:
- ori
- 载体来源:
- Scheich C, Kummel D, Soumailakakis D, Heinemann U, Bussow K.
pQLinkHD 载体图谱
质粒操作方法
1. 发货形式：质粒干粉(常温运输，存于-20度，请务必先转化提质粒后使用)
2. 收到质粒干粉后请先5000rpm离心1min，再加入20μl ddH2O溶解质粒;（质粒复测的浓度有时候与标称值差距较大，这可能是因为冻干质粒在管中的位置、复溶效率、测量偏差以及管壁的吸附导致，因此建议先转化提质粒后再使用）
3. 取1支100μl 感受态于冰上解冻10min，加入2μl质粒，再冰浴30min后，42℃热激60s，不要搅动，再冰浴2min；
4. 加入900μl无抗的LB液体培养基，180rpm震荡37℃培养45min (30℃培养1-1.5小时);
5. 6000rpm离心5min，仅留100μl上清液重悬细菌沉淀，并涂布至目标质粒抗性的LB平板上;
6. 将平板倒置37℃培养14h，如果要求是30℃则培养20h; (菌落过多则将质粒稀释后再转化；没有菌落则加入10μl质粒转化；建议不要直接转表达感受态， 要先转克隆感受态，重提质粒后再导入表达感受态);
7. 挑取单菌落至LB液体培养基中，加入对应抗生素，220rpm震荡培养14h，根据实验需要和质粒提取试剂盒说明书提取质粒。
pQLinkHD 载体序列
LOCUS 40924_36228 7062 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pQLinkHD, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7062)
AUTHORS Scheich C, Kummel D, Soumailakakis D, Heinemann U, Bussow K.
TITLE Vectors for co-expression of an unrestricted number of proteins
JOURNAL Nucleic Acids Res. 35 (6), E43 (2007)
PUBMED 17311810
REFERENCE 2 (bases 1 to 7062)
AUTHORS Buessow K, Scheich C.
TITLE Direct Submission
JOURNAL Submitted (25-SEP-2006) Vertebrate Genomics, Max Planck Institute
for Molecular Genetics, Ihnestr. 63-73, Berlin 14195, Germany
REFERENCE 3 (bases 1 to 7062)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 7062)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
Acids Res."; date: "2007"; volume: "35"; issue: "6"; pages: "E43"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(25-SEP-2006) Vertebrate Genomics, Max Planck Institute for
Molecular Genetics, Ihnestr. 63-73, Berlin 14195, Germany"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..7062
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 7..27
/label=LIC cassette with PacI restriction site
/note="LIC cassette with PacI restriction site"
promoter 36..80
/label=T5 promoter
/note="bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator"
protein_bind 88..104
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 123..134
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
regulatory 127..133
/regulatory_class="ribosome_binding_site"
misc_feature 141
/note="translation start site for inserts cloned into the
multiple cloning site"
CDS 147..167
/label=7xHis
/note="6xHis affinity tag"
protein_bind 180..304
/label=attR1
/note="recombination site for the Gateway(R) LR reaction"
CDS complement(736..1038)
/label=ccdB
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
CDS complement(1386..2042)
/label=CmR
/note="chloramphenicol acetyltransferase"
promoter complement(2043..2145)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
protein_bind complement(2323..2447)
/label=attR2
/note="recombination site for the Gateway(R) LR reaction"
terminator 2469..2563
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
misc_feature 2568..2617
/label=LIC cassette with SwaI, PacI restriction sites
/note="LIC cassette with SwaI, PacI restriction sites"
CDS 2657..3313
/label=CmR
/note="chloramphenicol acetyltransferase"
terminator 3381..3467
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
protein_bind complement(3526..3547)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
CDS complement(3563..4642)
/label=lacI
/note="lac repressor"
promoter complement(4643..4720)
/label=lacIq promoter
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
misc_feature 4912..5052
/label=bom
/note="basis of mobility region from pBR322"
rep_origin 5236
/label=ColE1 ori
/note="ColE1 ori"
rep_origin complement(5238..5826)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6000..6857)
/label=AmpR
/note="beta-lactamase"
promoter complement(6858..6962)
/label=AmpR promoter