pBCRE 载体 (V009244#)

载体基本信息
载体名称 pBCRE 载体抗性 Kanamycin
载体长度 14147 bp 载体类型 Binary vector
载体来源 Ma BG, Ma C, Niu JX.

pBCRE 载体载体图谱

pBCRE14147 bp7001400210028003500420049005600630070007700840091009800105001120011900126001330014000similar to traF in GenBank Accession Number X54459similar to tetA in GenBank Accession Number X75761T-DNA right borderNOSnptIINOSCAP binding sitelac promoterlac operatorM13 revCaMV 35SSK primercreNOSM13 fwdT-DNA left bordersimilar to tetA in GenBank Accession Number L13842similar to trfA in GenBank Accession Number X00713similar to NPTIII gene in GenBank Accession Number V01547similar to transposable element IS1 in GenBank Accession Number X58999similarity to NPT III gene in GenBank Accession Number V01547similar to kilA in GenBank Accession Number M62846ori V; similar to sequence in GenBank Accession Number M20134

pBCRE 载体载体序列

LOCUS       Exported               14147 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pBCRE, complete sequence.
ACCESSION   EU327976
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14147)
  AUTHORS   Ma BG, Ma C, Niu JX.
  TITLE     Gene expression controlled by inducible Cre/loxP recombination 
            system in transgenic tobacco
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14147)
  AUTHORS   Ma BG, Ma C, Niu JX.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2007) Department of Horticulture, Agriculture 
            College Shihezi University, Shihezi, Xinjiang 832000, P.R. China
REFERENCE   3  (bases 1 to 14147)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..14147
                     /organism="Binary vector pBCRE"
                     /mol_type="other DNA"
                     /db_xref="taxon:490502"
     misc_feature    complement(13..796)
                     /label=similar to traF in GenBank Accession Number X54459
                     /note="similar to traF in GenBank Accession Number X54459"
     CDS             complement(145..516)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            549..658
                     /note="incP origin of transfer"
     rep_origin      complement(790..1168)
                     /direction=LEFT
                     /note="ColE1 ori; similar to sequence in GenBank Accession 
                     Number V00268"
     misc_feature    complement(1161..2344)
                     /label=similar to tetA in GenBank Accession Number X75761
                     /note="similar to tetA in GenBank Accession Number X75761"
     CDS             complement(1339..1989)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    complement(2454..2478)
                     /label=T-DNA right border
                     /note="T-DNA right border"
     regulatory      2519..2825
                     /regulatory_class="promoter"
                     /note="NOS"
     promoter        2602..2781
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2634..2817
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2838..3632
                     /codon_start=1
                     /gene="nptII"
                     /product="neomycin phosphotransferase II"
                     /label=nptII
                     /protein_id="ABY50074.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            2838..3632
                     /gene="nptII"
                     /label=nptII
     regulatory      4022..4277
                     /regulatory_class="terminator"
                     /note="NOS"
     terminator      4022..4274
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4823..4844
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4859..4889
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4897..4913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4921..4937
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      4974..5808
                     /regulatory_class="promoter"
                     /note="CaMV 35S"
     promoter        5461..5806
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5815..5831
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5929..6960
                     /codon_start=1
                     /gene="cre"
                     /product="recombinase"
                     /label=cre
                     /protein_id="ABY50075.1"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRTLDSETGAMVRLLE
                     DGD"
     gene            5929..6960
                     /gene="cre"
                     /label=cre
     regulatory      7116..7368
                     /regulatory_class="terminator"
                     /note="NOS"
     terminator      7116..7368
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(7377..7393)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(8010..8035)
                     /label=T-DNA left border
                     /note="T-DNA left border"
     misc_feature    8011..8035
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    complement(8545..9587)
                     /label=similar to tetA in GenBank Accession Number L13842
                     /note="similar to tetA in GenBank Accession Number L13842"
     misc_feature    complement(9588..11069)
                     /label=similar to trfA in GenBank Accession Number X00713
                     /note="similar to trfA in GenBank Accession Number X00713"
     CDS             complement(9631..10779)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    complement(11070..12062)
                     /note="similar to NPTIII gene in GenBank Accession Number 
                     V01547"
     CDS             complement(11078..11872)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    complement(12063..12832)
                     /note="similar to transposable element IS1 in GenBank 
                     Accession Number X58999"
     mobile_element  12064..12831
                     /mobile_element_type="insertion sequence:IS1"
                     /note="prokaryotic transposable element"
     misc_feature    complement(12833..13183)
                     /note="similarity to NPT III gene in GenBank Accession 
                     Number V01547"
     misc_feature    complement(13184..13455)
                     /label=similar to kilA in GenBank Accession Number M62846
                     /note="similar to kilA in GenBank Accession Number M62846"
     rep_origin      13454..18
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      complement(13530..14147)
                     /direction=LEFT
                     /note="ori V; similar to sequence in GenBank Accession 
                     Number M20134"

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