pAAV-RC 载体 (V009666#)

载体基本信息
载体名称 pAAV-RC 载体抗性 Ampicillin
载体长度 7327 bp 载体类型 Cloning vector
载体来源 Grafsky AJ III.

pAAV-RC 载体载体图谱

pAAV-RC7327 bp300600900120015001800210024002700300033003600390042004500480051005400570060006300660069007200SK primerFRTRep78VP1M13 fwdf1 oriAmpR promoterAmpRoriCAP binding sitelac promoterlac operatorM13 rev

pAAV-RC 载体载体序列

LOCUS       40924_3184        7327 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAAV-RC, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7327)
  AUTHORS   Grafsky AJ III.
  TITLE     pAAV-RC for AAV Helper-Free System
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7327)
  AUTHORS   Grafsky AJ III.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2001) Technical Services, Stratagene, 11011 N. 
            Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE   3  (bases 1 to 7327)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7327)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-APR-2001) Technical Services, Stratagene, 11011 N. Torrey Pines 
            Rd., La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     36..52
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    66..113
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             131..1993
                     /gene="Rep78"
                     /label=Rep78
                     /note="Protein Rep78 from Adeno-associated virus 2 (isolate
                     Srivastava/1982). Accession#: Q89268"
     CDS             2013..4217
                     /gene="VP1"
                     /label=VP1
                     /note="Capsid protein VP1 from Adeno-associated virus 2
                     (isolate Srivastava/1982). Accession#: P03135"
     primer_bind     complement(4548..4564)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(4705..5160)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5187..5291
                     /label=AmpR promoter
     CDS             5292..6149
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6323..6911
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    7199..7220
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7235..7265
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7273..7289
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7297..7313
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"

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