我们所展示的质粒图谱主要是从文献和开放数据库中收集而来，主要是为了方便研究工作，其中一小部分质粒进行了质量控制，可供科学家使用。
所有的产品都严格仅供科学研究使用，不能应用于临床试验，包括人体摄入、注射或外用的药理学用途。
确保质粒的关键元件正确，但是我们并不能保证实验结果。页面展示的图谱序列为理论序列，可能与测序结果不一致，请自行比对后确定是否满足要求。(如果测过序，本页面一般会提供下载)
开放数据库中的大多数载体序列都没有被完全测序。如果实际序列与参考序列的相似度超过99%，则将其视为正确。
由于科学研究是在探索未知，具有很大的不确定性，在任何情况下，我们都不承担超出质粒本身的额外经济损失或责任。
- 载体名称:
- pUDC082
- 载体抗性:
- Ampicillin
- 载体长度:
- 10005 bp
- 载体类型:
- Cloning vector
- 复制子:
- ori
- 宿主:
- Yeast
- 载体来源:
- Beekwilder J, van Rossum H.
- 启动子:
- GPD
pUDC082 载体图谱
质粒操作方法
1. 发货形式：质粒干粉(常温运输，存于-20度，请务必先转化提质粒后使用)
2. 收到质粒干粉后请先5000rpm离心1min，再加入20μl ddH2O溶解质粒;（质粒复测的浓度有时候与标称值差距较大，这可能是因为冻干质粒在管中的位置、复溶效率、测量偏差以及管壁的吸附导致，因此建议先转化提质粒后再使用）
3. 取1支100μl 感受态于冰上解冻10min，加入2μl质粒，再冰浴30min后，42℃热激60s，不要搅动，再冰浴2min；
4. 加入900μl无抗的LB液体培养基，180rpm震荡37℃培养45min (30℃培养1-1.5小时);
5. 6000rpm离心5min，仅留100μl上清液重悬细菌沉淀，并涂布至目标质粒抗性的LB平板上;
6. 将平板倒置37℃培养14h，如果要求是30℃则培养20h; (菌落过多则将质粒稀释后再转化；没有菌落则加入10μl质粒转化；建议不要直接转表达感受态， 要先转克隆感受态，重提质粒后再导入表达感受态);
7. 挑取单菌落至LB液体培养基中，加入对应抗生素，220rpm震荡培养14h，根据实验需要和质粒提取试剂盒说明书提取质粒。
pUDC082 载体序列
LOCUS V002349 10005 bp DNA circular SYN 18-DEC-2018
DEFINITION Exported.
ACCESSION V002349
VERSION V002349
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 10005)
AUTHORS Beekwilder J, van Rossum H.
TITLE Production of carotenoids in Saccharomyces cerevisiae by a
self-processing polyprotein and conversion to beta-ionone
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 10005)
AUTHORS Beekwilder J, van Rossum H.
TITLE Direct Submission
JOURNAL Submitted (13-SEP-2013) BU Bioscience, Plant Research International,
P.O.Box 619, Wageningen, Gelderland 6700AP, Netherlands
REFERENCE 3 (bases 1 to 10005)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 10005)
AUTHORS .
TITLE Direct Submission
COMMENT ##Assembly-Data-START##
Assembly Method :: IDBA v. 1
Coverage :: 160
Sequencing Technology :: Illumina
##Assembly-Data-END##
SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(13-SEP-2013) BU Bioscience, Plant Research International, P.O.Box
619, Wageningen, Gelderland 6700AP, Netherlands"
SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..10005
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 7..650
/label="GAP promoter"
/note="promoter for glyceraldehyde-3-phosphate
dehydrogenase; also known as the TDH3 promoter"
primer_bind 656..672
/label="SK primer"
/note="common sequencing primer, one of multiple similar
variants"
CDS 674..2692
/gene="crtYB"
/label="Bifunctional lycopene cyclase/phytoene synthase"
/note="Bifunctional lycopene cyclase/phytoene synthase from
Phaffia rhodozyma. Accession#: Q7Z859"
CDS 2699..2752
/label="T2A"
/note="2A peptide from Thosea asigna virus capsid protein"
misc_feature 2753..4495
/note="phytoene desaturase from Xanthophyllomyces
dendrorhous; Region: CrtI"
misc_feature 4496..4555
/label="Region: T2A ribosomal skipping sequence"
/note="Region: T2A ribosomal skipping sequence"
CDS 4502..4555
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label="T2A"
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 5091..5102
/label="Factor Xa site"
/note="Factor Xa recognition and cleavage site"
terminator 5748..5945
/label="TEF terminator"
/note="Ashbya gossypii TEF terminator"
CDS 6439..7242
/codon_start=1
/product="orotidine-5'-phosphate decarboxylase"
/label="orotidine-5'-phosphate decarboxylase"
/note="URA3; derived from Kluyveromyces lactis"
/protein_id="AHZ97959.1"
/translation="MSTKSYTSRAETHASPVASKLLRLMDEKKTNLCASLDVRSTDELL
KLVETLGPYICLLKTHVDILDDFSYEGTVVPLKALAEKYKFLIFEDRKFADIGNTVKLQ
YTSGVYRIAEWSDITNAHGVTGAGIVAGLKQGAQEVTKEPRGLLMLAELSSKGSLAHGE
YTKGTVDIAKSDKDFVIGFIAQNDMGGREEGFDWLIMTPGVGLDDKGDALGQQYRTVDE
VVSGGSDIIIVGRGLFAKGRDPKVEGERYRNAGWEAYQKRISAPH"
promoter 7426..7530
/label="AmpR promoter"
CDS 7531..8388
/label="AmpR"
/note="beta-lactamase"
rep_origin 8562..9150
/direction=RIGHT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature complement(9422..9925)
/label="CEN/ARS"
/note="S. cerevisiae CEN6 centromere fused to an
autonomously replicating sequence"