我们所展示的质粒图谱主要是从文献和开放数据库中收集而来，主要是为了方便研究工作，其中一小部分质粒进行了质量控制，可供科学家使用。
所有的产品都严格仅供科学研究使用，不能应用于临床试验，包括人体摄入、注射或外用的药理学用途。
确保质粒的关键元件正确，但是我们并不能保证实验结果。页面展示的图谱序列为理论序列，可能与测序结果不一致，请自行比对后确定是否满足要求。(如果测过序，本页面一般会提供下载)
开放数据库中的大多数载体序列都没有被完全测序。如果实际序列与参考序列的相似度超过99%，则将其视为正确。
由于科学研究是在探索未知，具有很大的不确定性，在任何情况下，我们都不承担超出质粒本身的额外经济损失或责任。
- 载体名称:
- pLH7500
- 载体抗性:
- Streptomycin
- 载体长度:
- 8903 bp
- 载体类型:
- Binary vector
- 复制子:
- ori
- 宿主:
- Plants
- 载体来源:
- Hausmann L, Toepfer R.
- 启动子:
- CaMV 35S
pLH7500 载体图谱
质粒操作方法
1. 发货形式：质粒干粉(常温运输，存于-20度，请务必先转化提质粒后使用)
2. 收到质粒干粉后请先5000rpm离心1min，再加入20μl ddH2O溶解质粒;（质粒复测的浓度有时候与标称值差距较大，这可能是因为冻干质粒在管中的位置、复溶效率、测量偏差以及管壁的吸附导致，因此建议先转化提质粒后再使用）
3. 取1支100μl 感受态于冰上解冻10min，加入2μl质粒，再冰浴30min后，42℃热激60s，不要搅动，再冰浴2min；
4. 加入900μl无抗的LB液体培养基，180rpm震荡37℃培养45min (30℃培养1-1.5小时);
5. 6000rpm离心5min，仅留100μl上清液重悬细菌沉淀，并涂布至目标质粒抗性的LB平板上;
6. 将平板倒置37℃培养14h，如果要求是30℃则培养20h; (菌落过多则将质粒稀释后再转化；没有菌落则加入10μl质粒转化；建议不要直接转表达感受态， 要先转克隆感受态，重提质粒后再导入表达感受态);
7. 挑取单菌落至LB液体培养基中，加入对应抗生素，220rpm震荡培养14h，根据实验需要和质粒提取试剂盒说明书提取质粒。
pLH7500 载体序列
LOCUS 40924_28257 8903 bp DNA circular SYN 18-DEC-2018
DEFINITION Binary vector pLH7500, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8903)
AUTHORS Hausmann L, Toepfer R.
TITLE Development of Plasmid Vectors
JOURNAL (in) Brauer,D., Roebbelen,G. and Toepfer,R. (Eds.); BIOENGINEERING
OF CUSTOM-TAILORED RAPE VARIETIES: 155-172; GPZ e. V., Von
Sieboldstr. 8, Goettingen, Germany (1999)
REFERENCE 2 (bases 1 to 8903)
AUTHORS Hausmann L, Toepfer R.
TITLE Direct Submission
JOURNAL Submitted (12-FEB-2003) Institute for Grapevine Breeding
Geilweilerhof, Siebeldingen 76833, Germany
REFERENCE 3 (bases 1 to 8903)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 8903)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "(in)
Brauer,D., Roebbelen,G. and Toepfer,R. (Eds.)"; volume: "
BIOENGINEERING OF CUSTOM-TAILORED RAPE VARIETIES"; pages: " 155-172;
GPZ e. V., Von Sieboldstr. 8, Goettingen, Germany (1999"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(12-FEB-2003) Institute for Grapevine Breeding Geilweilerhof,
Siebeldingen 76833, Germany"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..8903
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 80..424
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
CDS 439..987
/label=BlpR
/note="phosphinothricin acetyltransferase"
regulatory 1013..1212
/note="terminator sequence from the 35S gene of Cauliflower
mosaic virus in GenBank Accession Numbers X05868 and
V00140"
/regulatory_class="terminator"
misc_difference 1051
/label=compared to GenBank Accession Number X05868
/note="compared to GenBank Accession Number X05868"
/experiment="experimental evidence, no additional details
recorded"
regulatory 1178..1193
/regulatory_class="polyA_signal_sequence"
misc_feature 1208
/label=putative transcription stop site
/note="putative transcription stop site"
misc_feature 1231..1332
/note="multiple cloning site from vector pBlueSfi AB in
GenBank Accession Number AF327874"
regulatory 1302..1311
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
misc_difference 1358
/replace="a"
/label=compared to GenBank Accession Number X07435
/note="compared to GenBank Accession Number X07435"
/experiment="experimental evidence, no additional details
recorded"
misc_feature 1377..1401
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
repeat_region 1425..1437
/label=repeat C'
/note="repeat C'"
misc_difference 1431
/label=compared to GenBank Accession Number X07435
/note="compared to GenBank Accession Number X07435"
/experiment="experimental evidence, no additional details
recorded"
repeat_region 1460..1471
/label=repeat C
/note="repeat C"
repeat_region 1485..1495
/label=repeat B
/note="repeat B"
repeat_region 1512..1522
/label=repeat B
/note="repeat B"
misc_difference 1517
/label=compared to GenBank Accession Number X07435
/note="compared to GenBank Accession Number X07435"
/experiment="experimental evidence, no additional details
recorded"
misc_difference 1528
/replace="a"
/label=compared to GenBank Accession Number X07435
/note="compared to GenBank Accession Number X07435"
/experiment="experimental evidence, no additional details
recorded"
misc_feature complement(1545..1935)
/note="3' sequence of the ornithine cyclodeaminase gene
ocd; non-functional"
misc_difference 1721
/replace="t"
/label=compared to GenBank Accession Number X07435
/note="compared to GenBank Accession Number X07435"
/experiment="experimental evidence, no additional details
recorded"
rep_origin 2046..2634
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_difference 2732..2733
/replace="gta"
/label=compared to GenBank Accession Number J01749
/note="compared to GenBank Accession Number J01749"
/experiment="experimental evidence, no additional details
recorded"
misc_feature complement(2819..2959)
/label=bom
/note="basis of mobility region from pBR322"
regulatory 3247..3252
/regulatory_class="minus_35_signal"
regulatory 3273..3278
/regulatory_class="minus_10_signal"
regulatory 3295..3299
/regulatory_class="ribosome_binding_site"
CDS 3309..3995
/codon_start=1
/product="resolvase"
/label=resolvase
/note="resolvase-like protein encoded by parR of plasmid
pVS1; similar to Tn3 resolvase in GenBank Accession Number
V00613, Tn917 resolvase in GenBank Accession Number M11180,
and RK2 ParA in GenBank Accession Number L27758"
/protein_id="AAO85366.1"
/translation="MNKSAAAGLLGYARVSTDDQDLTNQRAELHAAGCTKLFSEKITGT
RRDRPELARMLDHLRPGDVVTVTRLDRLARSTRDLLDIAERIQEAGAGLRSLAEPWADT
TTPAGRMVLTVFAGIAEFERSLIIDRTRSGREAAKARGVKFGPRPTLTPAQIAHARELI
DQEGRTVKEAAALLGVHRSTLYRALERSEEVTPTEARRRGAFREDALTEADALAAAENE
RQEEQA"
misc_difference 3728
/replace="c"
/note="original nucleotide changed to destroy a SfiI
restriction site"
/experiment="experimental evidence, no additional details
recorded"
stem_loop 4139..4171
/label=palindromic sequence
/note="palindromic sequence"
misc_difference 4147
/replace="g"
/note="nucleotide changed to conserve palindromic sequence
following SfiI restriction site-destruction"
/experiment="experimental evidence, no additional details
recorded"
misc_difference 4162
/replace="c"
/note="original nucleotide changed to destroy two SfiI
restriction sites"
/experiment="experimental evidence, no additional details
recorded"
regulatory 4229..4234
/regulatory_class="minus_35_signal"
regulatory 4250..4255
/regulatory_class="minus_10_signal"
regulatory 4283..4290
/regulatory_class="ribosome_binding_site"
CDS 4294..4920
/label=pVS1 StaA
/note="stability protein from the Pseudomonas plasmid pVS1
(Heeb et al., 2000)"
misc_feature 5190..5202
/note="identical to the korB operator binding site of
plasmid RK2 in GenBank Accession Number L27758.1"
CDS 5352..6422
/label=pVS1 RepA
/note="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
stem_loop 6439..6461
/label=palindromic sequence
/note="palindromic sequence"
rep_origin 6491..6685
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
repeat_region 6782..6795
/label=repeat E
/rpt_unit_range=6782 .. 6788
/note="repeat E"
regulatory 7026..7031
/regulatory_class="minus_35_signal"
regulatory 7049..7054
/regulatory_class="minus_10_signal"
CDS 7290..8078
/label=SmR
/note="aminoglycoside adenylyltransferase (Murphy, 1985)"
stem_loop 8084..8141
/note="palindromic sequence; putative termination signal"
misc_difference 8318..8319
/replace="ggtacc"
/note="compared to GenBank Accession Number X00493; removed
to destroy a KpnI restriction site"
misc_feature 8601..8625
/label=LB T-DNA repeat
/note="left border repeat from octopine Ach5 T-DNA"