pd1EGFP-N1 载体 (V012060#)

载体基本信息
载体名称 pd1EGFP-N1 载体抗性 Kanamycin
载体长度 4858 bp 载体类型 Fluorescent Protein Genes & Plasmids
载体来源 Clontech 筛选标记 Neomycin/G418(Geneticin)
拷贝数 High copy number

pd1EGFP-N1 载体载体图谱

pd1EGFP-N14858 bp6001200180024003000360042004800CMV enhancerCMV promoterMCSd1EGFPSV40 poly(A) signalf1 oriAmpR promoterSV40 promoterNeoR/KanRHSV TK poly(A) signalori

pd1EGFP-N1 载体载体序列

LOCUS       Exported                4858 bp ds-DNA    circular SYN 26-11-2013
DEFINITION  Vector for fusing destabilized EGFP, with an in vivo half-life of ~1
            hour, to the C-terminus of a partner protein.
ACCESSION   .
VERSION     .
KEYWORDS    pd1EGFP-N1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Clontech
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     enhancer        61..364
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    591..671
                     /note="MCS"
                     /note="multiple cloning site of fluorescent protein 
                     plasmids"
     CDS             679..1524
                     /codon_start=1
                     /product="EGFP destabilized by a mutated version of 
                     residues 422-461 of mouse ornithine decarboxylase"
                     /note="d1EGFP"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKKLSHGFPPAVAAQDDGTLPMSCAQESGMDRHPAACASARINV
                     "
     polyA_signal    1646..1767
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1774..2229)
                     /direction=LEFT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2256..2360
                     /gene="bla"
                     /note="AmpR promoter"
     promoter        2362..2719
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      2570..2705
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     CDS             2754..3548
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    3780..3827
                     /note="HSV TK poly(A) signal"
                     /note="herpesvirus thymidine kinase polyadenylation signal"
     rep_origin      4156..4744
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

我们不提供该载体,载体信息仅供查询和参考。