- 载体名称:
- pGint
- 载体抗性:
- Kanamycin
- 载体长度:
- 4804 bp
- 载体类型:
- Mammalian Expression
- 复制子:
- ori
- 筛选标记:
- Neomycin (select with G418)
- 拷贝数:
- High Copy
- 启动子:
- CMV
- 克隆方法:
- Restriction Enzyme
- 5'引物:
- commercial, from Clontech
pGint 载体载体图谱
pGint 载体载体序列
LOCUS 40924_21888 4804 bp DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4804) AUTHORS Bonano VI, Oltean S, Garcia-Blanco MA TITLE A protocol for imaging alternative splicing regulation in vivo using fluorescence reporters in transgenic mice. JOURNAL Nat Protoc. 2007 . 2(9):2166-81. PUBMED 17853873 REFERENCE 2 (bases 1 to 4804) TITLE Direct Submission REFERENCE 3 (bases 1 to 4804) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat Protoc. 2007 . 2(9):2166-81." COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..4804 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 61..364 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 365..568 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" regulatory 619..628 /label=Kozak sequence /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" primer_bind complement(670..691) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(1056..1075) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1403..1424 /label=EGFP-C /note="EGFP, forward primer" polyA_signal 1592..1713 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin complement(1720..2175) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 2202..2306 /label=AmpR promoter promoter 2308..2665 /label=SV40 promoter /note="SV40 enhancer and early promoter" CDS 2700..3491 /codon_start=1 /label=NeoR/KanR /note="aminoglycoside phosphotransferase" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3682..3701) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 3726..3773 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4102..4690 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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