- 载体名称:
- pBOMB4-MCI
- 载体抗性:
- Ampicillin
- 载体长度:
- 10575 bp
- 载体类型:
- Cloning vector
- 复制子:
- ori
- 载体来源:
- Bauler LD, Hackstadt T.
pBOMB4-MCI 载体载体图谱
pBOMB4-MCI 载体载体序列
LOCUS 40924_6971 10575 bp DNA circular SYN 17-DEC-2018 DEFINITION Cloning vector pBOMB4-MCI, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10575) AUTHORS Bauler LD, Hackstadt T. TITLE Expression and Targeting of Secreted Proteins from Chlamydia trachomatis JOURNAL J. Bacteriol. 196 (7), 1325-1334 (2014) PUBMED 24443531 REFERENCE 2 (bases 1 to 10575) AUTHORS Bauler LD, Hackstadt T. TITLE Direct Submission JOURNAL Submitted (30-OCT-2013) Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, NIAID, NIH, 903 S. Fourth St., Hamilton, MT 59840, USA REFERENCE 3 (bases 1 to 10575) TITLE Direct Submission REFERENCE 4 (bases 1 to 10575) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J. Bacteriol."; date: "2014"; volume: "196"; issue: "7"; pages: "1325-1334" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (30-OCT-2013) Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, NIAID, NIH, 903 S. Fourth St., Hamilton, MT 59840, USA" COMMENT SGRef: number: 3; type: "Journal Article" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..10575 /mol_type="other DNA" /organism="synthetic DNA construct" CDS complement(55..969) /note="Virulence plasmid integrase pGP7-D from Chlamydia trachomatis. Accession#: P0CE19" misc_feature 7555..7589 /label=multiple cloning site /note="multiple cloning site" promoter 7590..7661 /label=AmpR promoter CDS 7662..8519 /label=AmpR /note="beta-lactamase" rep_origin 8691..9275 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS 9811..10518 /label=mCherry /note="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)"
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