- 载体名称:
- mTert-pBabe-puro
- 载体抗性:
- Ampicillin
- 载体长度:
- 7820 bp
- 载体类型:
- Mammalian Expression, Retroviral
- 复制子:
- ori
- 筛选标记:
- Puromycin
- 拷贝数:
- High Copy
- 启动子:
- LTR
- 克隆方法:
- Restriction Enzyme
- 5'引物:
- mTERT 1: 5′-TTCGAACAGCAAACCAACAG-3′
mTert-pBabe-puro 载体载体图谱
mTert-pBabe-puro 载体载体序列
LOCUS 40924_2134 7820 bp DNA circular SYN 13-MAY-2021
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7820)
AUTHORS Alvarez MB, Childress P, Philip BK, Gerard-O'Riley R, Hanlon M,
Herbert BS, Robling AG, Pavalko FM, Bidwell JP
TITLE Immortalization and characterization of osteoblast cell lines
generated from wild-type and Nmp4-null mouse bone marrow stromal
cells using murine telomerase reverse transcriptase (mTERT).
JOURNAL J Cell Physiol. 2012 May;227(5):1873-82. doi: 10.1002/jcp.22915.
PUBMED 21732358
REFERENCE 2 (bases 1 to 7820)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 7820)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; doi: "10.1002/jcp";
journalName: "J Cell Physiol"; date: "2012-05"; volume: "227";
issue: "5"; pages: "1873-82"
COMMENT SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..7820
/mol_type="other DNA"
/organism="synthetic DNA construct"
LTR 8..477
/label=LTR
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 542..741
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 942..1358
/codon_start=1
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
/translation="GQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTF
NVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKP
PPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
promoter 4155..4484
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 4494..5090
/codon_start=1
/label=PuroR
/note="puromycin N-acetyltransferase"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
LTR 5192..5783
/label=LTR
/note="long terminal repeat from Moloney murine leukemia
virus"
primer_bind complement(5913..5930)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6084..6672)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6835..7692)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(7693..7797)
/label=AmpR promoter
我们不提供该载体，载体信息仅供查询和参考。