- 载体名称:
- pNI2
- 载体抗性:
- Tetracycline
- 载体长度:
- 3111 bp
- 载体类型:
- Gene knockout
- 复制子:
- ori
- 宿主:
- E. coli
- 启动子:
- tet
pNI2 载体载体图谱
pNI2 载体载体序列
LOCUS 62056_17980 3111 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3111) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..3111 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 196..224 /label=tet promoter /note="E. coli promoter for tetracycline efflux protein gene" CDS 272..1459 /codon_start=1 /label=TcR /note="tetracycline efflux protein" /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST" promoter complement(1637..1655) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1660..1676) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" rep_origin 1960..2548 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" terminator complement(2878..2905) /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator complement(2997..3083) /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene"
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