- 载体名称:
- pAB149
- 载体抗性:
- Ampicillin
- 载体长度:
- 6570 bp
- 载体类型:
- Cloning vector
- 复制子:
- ori
- 载体来源:
- Scheller L, Schmollack M, Bertschi A, Mansouri M
pAB149 载体载体图谱
pAB149 载体载体序列
LOCUS 62056_2640 6570 bp DNA circular SYN 30-JUN-2020 DEFINITION Cloning vector pAB149, complete sequence. ACCESSION MT267299 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6570) AUTHORS Scheller L, Schmollack M, Bertschi A, Mansouri M, Saxena P, Fussenegger M. TITLE Phosphoregulated orthogonal signal transduction in mammalian cells JOURNAL Nat Commun 11 (1), 3085 (2020) PUBMED 32555187 REFERENCE 2 (bases 1 to 6570) AUTHORS Scheller L. TITLE Direct Submission JOURNAL Submitted (29-MAR-2020) STI IBI-STI LPDI, EPFL, AI 3123 (Batiment AI), Station 19, Lausanne 1015, Switzerland REFERENCE 3 (bases 1 to 6570) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun"; date: "2020"; volume: "11"; issue: "1"; pages: "3085" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (29-MAR-2020) STI IBI-STI LPDI, EPFL, AI 3123 (Batiment AI), Station 19, Lausanne 1015, Switzerland" FEATURES Location/Qualifiers source 1..6570 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 40..419 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 420..623 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" promoter 668..686 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" misc_feature 715..720 /label=Kozak+ATG /note="Kozak+ATG" CDS 739..1455 /gene="dcuR" /label=dcuR /note="Transcriptional regulatory protein DcuR from Escherichia coli (strain K12). Accession#: P0AD01" CDS 1492..2109 /label=TetR /note="tetracycline repressor TetR" polyA_signal 2170..2394 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 2440..2868 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 2882..3211 /label=SV40 promoter /note="SV40 enhancer and early promoter" CDS 3278..4069 /label=NeoR/KanR /note="aminoglycoside phosphotransferase" polyA_signal 4246..4379 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4416..4432) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(4440..4456) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4464..4494) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(4509..4530) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(4818..5403) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(5577..6434) /label=AmpR /note="beta-lactamase" promoter complement(6435..6539) /label=AmpR promoter
我们不提供该载体,载体信息仅供查询和参考。