- 载体名称:
- pET21a(+)-Bst LF-6xHis
- 载体抗性:
- Ampicillin
- 载体长度:
- 7133 bp
- 载体类型:
- Protein expression
- 复制子:
- ori
- 宿主:
- E. coli
- 启动子:
- T7
- 5'引物:
- T7:TAATACGACTCACTATAGGG
pET21a(+)-Bst LF-6xHis 载体载体图谱
pET21a(+)-Bst LF-6xHis 载体载体序列
LOCUS 62056_9725 7133 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7133) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..7133 /mol_type="other DNA" /organism="synthetic DNA construct" promoter complement(602..679) /label=lacI promoter promoter 988..1006 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 1007..1031 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 1046..1068 /label=RBS /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 2852..2869 /codon_start=1 /label=6xHis /note="6xHis affinity tag" /translation="HHHHHH" terminator 2936..2983 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" rep_origin 3020..3475 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 3501..3605 /label=AmpR promoter CDS 3606..4463 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 4637..5225 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" misc_feature complement(5411..5550) /label=bom /note="basis of mobility region from pBR322" CDS complement(5655..5843) /codon_start=1 /label=rop /note="Rop protein, which maintains plasmids at low copy number" /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA DELYRSCLARFGDDGENL" protein_bind complement(6618..6639) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." CDS complement(join(6655..7133,1..601)) /codon_start=1 /label=lacI /note="lac repressor" /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ"
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