The pGEX-6P-1 is a bacterial vector for expressing GST fusion proteins with a PreScission protease site. For other reading frames, use pGEX-6P-2 or pGEX-6P-3.
- 载体名称:
- pGEX-6P-1
- 载体抗性:
- Ampicillin
- 载体长度:
- 4984 bp
- 载体类型:
- pGEX Vectors (GE Healthcare)
- 复制子:
- ori
- 载体来源:
- GE Healthcare
- 拷贝数:
- High copy number
- 启动子:
- Tac
- 5'引物:
- GGGCTGGCAAGCCACGTTTGGTG
- 3'引物:
- CCGGGAGCTGCATGTGTCAGAGG
- 载体标签:
- N-GST
pGEX-6P-1 载体载体图谱
pGEX-6P-1 载体载体序列
LOCUS pGEX-6P-1. 4984 bp DNA circular SYN 01-JAN-1980 DEFINITION Bacterial vector for expressing GST fusion proteins with a PreScission protease site. For other reading frames, use pGEX-6P-2 or pGEX-6P-3. ACCESSION . VERSION . KEYWORDS pGEX-6P-1. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4984) AUTHORS GE Healthcare TITLE Direct Submission REFERENCE 2 (bases 1 to 4984) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..4984 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 183..211 /label=tac promoter /note="strong E. coli promoter; hybrid between the trp and lac UV5 promoters" protein_bind 219..235 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." CDS 258..911 /label=GST /note="glutathione S-transferase from Schistosoma japonicum" CDS 918..941 /label=HRV 3C site /note="recognition and cleavage site for human rhinovirus 3C and PreScission proteases" misc_feature 940..981 /label=MCS /note="MCS" /note="multiple cloning site" misc_feature 986..996 /label=stop codons /note="stop codons" /note="stop codons in all three reading frames" promoter 1287..1391 /label=AmpR promoter CDS 1392..2249 /label=AmpR /note="beta-lactamase" rep_origin 2423..3011 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" promoter 3255..3332 /label=lacIq promoter /note="In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold." CDS 3333..4412 /label=lacI /note="lac repressor" protein_bind 4428..4449 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 4464..4494 /label=lac promoter /note="promoter for the E. coli lac operon" CDS 4538..4711 /label=lacZ-alpha /note="LacZ-alpha fragment of beta-galactosidase"
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