Introducing the recombinant E3 ubiquitin-protein ligase ZFP91 fragment (residues 309-466), a stable and soluble protein unit designed for structural and biochemical studies. This fragment supports research into the non-canonical NF-kappa-B2 pathway and cell proliferation mechanisms, offering a reliable tool for life science investigations.

E3 ubiquitin-protein ligase ZFP91 (EC 2.3.2.27) is an atypical RING-type E3 ubiquitin transferase that plays a critical role in the non-canonical NF-kappa-B2/NFKB2 pathway. It mediates 'Lys-63'-linked ubiquitination of MAP3K14/NIK, leading to stabilization and activation of MAP3K14/NIK, and may also contribute to cell proliferation and anti-apoptotic processes. The full-length protein is localized in the nucleus, as supported by experimental evidence. This recombinant fragment (309-466) was selected to provide a stable and soluble region for detailed analysis of ZFP91's functional mechanisms.