- 抗体类型:多克隆
- 抗体来源:兔
- 抗体应用:ELISA, WB, IHC, IF
- 特异性:Human,Mouse,Rat; other species not tested.
产品详情
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产品名称
YME1L1 antibody
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抗体类型
多克隆
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抗体来源
兔
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抗体亚型
兔IgG
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抗体描述
YME1L1 Rabbit Polyclonal antibody. Positive WB detected in mouse heart tissue. Positive IHC detected in human colon cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 86kd
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抗体应用
ELISA, WB, IHC, IF
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应用推荐
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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特异性
Human,Mouse,Rat; other species not tested.
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蛋白别名
FTSH, FTSH1, Meg 4, MEG4, PAMP, YME1 like 1 (S. cerevisiae), YME1 like protein 1, YME1L, YME1L1
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制备方法
This antibody was obtained by immunization of YME1L1 recombinant protein (Accession Number: NM_014263). Purification method: Antigen affinity purified.
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组分
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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储存方法
Store at -20℃. DO NOT ALIQUOT
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背景介绍
YME1L1(ATP-dependent zinc metalloprotease) is also named as FTSH1, YME1L,Meg-4,PAMP. YME1L1 plays a phylogenetically conserved role in mitochondrial protein metabolism.It also ensures cell proliferation, maintains normal cristae morphology and complex I respiration activity, promotes antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins. It has 3 isoforms produced by alternative splicing with the molecular weight of 86 kDa, 80 kDa and 76 kDa. This protein can migrate with a molecular weight of about 55-60 kDa, which is the size of the mature YME1L1 protein(PMID:22252130; 22354088).
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参考文献
- Mishra P, Carelli V, Manfredi G, Chan DC. Proteolytic cleavage of Opa1 stimulates mitochondrial inner membrane fusion and couples fusion to oxidative phosphorylation. Cell metabolism. 19(4):630-41. 2014.
- Ruan Y, Li H, Zhang K, Jian F, Tang J, Song Z. Loss of Yme1L perturbates mitochondrial dynamics. Cell death & disease. 4:e896. 2013.
- Richter-Dennerlein R, Korwitz A, Haag M. DNAJC19, a mitochondrial cochaperone associated with cardiomyopathy, forms a complex with prohibitins to regulate cardiolipin remodeling. Cell metabolism. 20(1):158-71. 2014.
- Baburamani AA, Hurling C, Stolp H. Mitochondrial Optic Atrophy (OPA) 1 Processing Is Altered in Response to Neonatal Hypoxic-Ischemic Brain Injury. International journal of molecular sciences. 16(9):22509-26. 2015.
- Li H, Ruan Y, Zhang K. Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization. Cell death and differentiation. 23(3):380-92. 2016.
- Richter U, Lahtinen T, Marttinen P, Suomi F, Battersby BJ. Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness. The Journal of cell biology. 211(2):373-89. 2015.
- Ehses S, Raschke I, Mancuso G. Regulation of OPA1 processing and mitochondrial fusion by m-AAA protease isoenzymes and OMA1. The Journal of cell biology. 187(7):1023-36. 2009.
- Hornig-Do HT, Tatsuta T, Buckermann A. Nonsense mutations in the COX1 subunit impair the stability of respiratory chain complexes rather than their assembly. The EMBO journal. 31(5):1293-307. 2012.