Recombinant Human V-type proton ATPase subunit F (ATP6V1F) fragment (residues 1-119) is a high-quality, stable protein designed for structural and biochemical studies. This fragment represents a soluble and functional unit of the V-ATPase complex, ideal for investigating proton pump mechanisms and intracellular acidification processes.

V-type proton ATPase subunit F is a key component of the V1 complex in vacuolar (H+)-ATPase (V-ATPase), a multisubunit enzyme that hydrolyzes ATP to drive proton translocation across membranes. V-ATPase plays a critical role in acidifying intracellular compartments, such as cytoplasmic vesicles, secretory vesicles, synaptic vesicles, and clathrin-coated vesicles, and may also function at the plasma membrane to modulate extracellular pH. This enzyme is essential for maintaining pH homeostasis in cellular environments, impacting processes like protein sorting, neurotransmitter uptake, and organelle function. The recombinant fragment (residues 1-119) encompasses the full