重组大鼠UCHL1蛋白

大鼠

大肠杆菌

Glu 2 - Ala 223

A DNA sequence encoding the rat UCHL1 (Q00981) (Glu 2-Ala 223) was fused with a polyhistidine tag at the N-terminus.

> 97 % as determined by SDS-PAGE

Measured by the hydrolysis of UbiquitinAMC. The specific activity is >100 pmoles/min/μg.

The recombinant rat UCHL1 comprises 233 amino acids and predicts a molecular mass of 26.2 kDa. The apparent molecular mass of the rat UCHL1 is approximately 28 kDa in SDS-PAGE under reducing conditions.

GAD; UCHL-1; UCHL1; R75593; HEL-117; UCH-L1; C88048; PARK5; PGP9.5; PGP95; PGP 9.5; AW822034; NDGOA

Ubiquitin carboxyl-terminal hydrolase isozyme L1

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity (By similarity).

Lyophilized from sterile PBS, pH 7.4
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

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Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.