Thymosin Alpha 1, purity >98% 多肽

Ac-SDAAVDTSSEITTKDLKEKKEVVEEAEN-OH

Ac-Ser-Asp-Ala-Ala-Val-Asp-Thr-Ser-Ser-Glu-Ile-Thr-Thr-Lys-Asp-Leu-Lys-Glu-Lys-Lys-Glu-Val-Val-Glu-Glu-Ala-Glu-Asn-OH

28

98.17

C₁₂₉H₂₁₅N₃₃O₅₅

3108.25

固相合成

Thymic Hormones and Their Role in Aging. The increase in the average life span of humans during the last century has been accompanied by a parallel increase in a number of diseases associated with aging, including cancer, and certain infectious and autoimmune diseases. The decline of immunocompetence with aging has been correlated with the increased incidence of these diseases and extensive reviews describing the changes in the immune system during the aging process has recently been published. Although the decline of the immune system is accompanied by alterations in all its compartments, the most affected appears to be the T-cell compartment. Decline in T cell mitogen-induced proliferation, allogeneic mixed leukocyte reaction (MLR), cytotoxic T lymphocyte responses (CTE), helper T-cell activity, IL2 production, IL2 receptor expression, calcium uptake, PNP activity (PNPpurine nucleoside phosphorylase), adenylate cyclase activity, etc have been observed in T-cells from aging individuals and animals. These changes lead to an increased susceptibility to infections, decrease resistance to tumor growth, and an increased incidence of autoimmune diseases, probably secondary to an immunologic imbalar~ce resulting in an increased response towards self antigens, characterized by a rise in the frequency of autoantibodies observed in aged individuals.

一般情况下， Thymosin Alpha 1, purity >98% 多肽 粉末应该保存在-20甚至-70摄氏度. Thymosin Alpha 1, purity >98% 多肽 溶解后应该-70摄氏度分装保存，避免反复冻融. 了解更多细节，请查阅手册:多肽溶解及保存指南

• Sztein MB, Goldstein AL. Thymic hormones--a clinical update. Springer Semin Immunopathol. 1986;9(1):1-18. doi: 10.1007/BF00201901. PMID: 3014672.
• Dominari A, Hathaway Iii D, Pandav K, Matos W, Biswas S, Reddy G, Thevuthasan S, Khan MA, Mathew A, Makkar SS, Zaidi M, Fahem MMM, Beas R, Castaneda V, Paul T, Halpern J, Baralt D. Thymosin alpha 1: A comprehensive review of the literature. World J Virol. 2020 Dec 15;9(5):67-78. doi: 10.5501/wjv.v9.i5.67. PMID: 33362999; PMCID: PMC7747025.

三氟乙酸（Trifluoroacetic acid，简称 TFA）是高效液相色谱（简称 HPLC）纯化过程中常用的反离子。TFA 的存在可能会对肽的净重量、外观及溶解度产生影响，具体如下：

对净重量的影响：TFA 盐会增加产物的总质量。在大多数情况下，肽的含量占总重量的 80% 以上，剩余部分则为 TFA。

对溶解度的影响：TFA 盐通常能提高肽在水溶液中的溶解度。

在生物检测中的影响：对于大多数标准的体外检测，残留的 TFA 水平一般不会造成干扰。但需注意，在高灵敏度的细胞研究或生化研究中，需关注其存在可能带来的影响。

干品多肽的重量中不仅仅包含多肽，还包含有一些非肽的组份，如水、被吸收的溶剂、配位离子和盐等。肽的净含量是指肽在其中的重量百分比，这个百分比的数值范围很大，可能从50%到90%，取决于纯度、序列以及合成和纯化的方法，不要将肽的净含量和肽的纯度混为一谈，他们是两个完全不同的概念。纯度通常是通过HPLC测定的。纯度定义的是多肽样品中含正确序列的组分的百分比，而肽的净含量是指样品中肽类物质相对于总物质所占的百分比，肽的净含量通常是氨基酸组分分析或紫外分光法测定的，这个信息主要是在一些对肽的浓度很敏感的实验中，对计算肽的浓度是很重要的。