重组人TGFBR2蛋白

人

人胚胎肾细胞（HEK293）

Met 1 - Asp 159

A DNA sequence encoding the extracellular domain (Met 1-Asp 159) of human TGF? receptor 2 (NP_003233.4) was expressed with the fused C-terminal His-tagged Fc region of human IgG1 at the C-terminus.

> 97 % as determined by SDS-PAGE

Measured by its ability to inhibit TGF-beta1 activity on Mv-1-lu mink lung epithelial cells.
The ED₅₀ for this effect is typically 0.2-3.0 μg/ml in the presence of 1 ng/mL of recombinant human TGF-beta1.

The recombinant human TGF? R2/Fc is a disulfide-linked homodimeric protein after removal of the signal peptide. The monomer consists of 383 amino acids and predicts a molecular mass of 43.4 kDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of this monomer is approximately 60-65 kDa due to the glycosylation.

DNIIR; LDS1B; TBR-II; LDS2; 1110020H15RIK; TGFBR2; TBETAR-II; TAAD2; TGFR-2; MFS2; RIIC; TGFBETA-RII; TBETARII; AU042018; AAT3; FAA3; RIIDN; LDS2B

TGF-beta receptor type-2

Transmembrane serine/threonine kinase forming with the TGF-beta type I serine/threonine kinase receptor, TGFBR1, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFRB1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways.

Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

< 1.0 EU per μg of the protein as determined by the LAL method

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.