Recombinant human Dimethyladenosine transferase 2 (TFB2M) fragment (residues 72-396), a stable and soluble unit ideal for investigating mitochondrial transcription initiation and rRNA methylation mechanisms in structural and biochemical studies.

Dimethyladenosine transferase 2 (TFB2M) is a mitochondrial protein that functions as an S-adenosyl-L-methionine-dependent rRNA methyltransferase, likely methylating specific adenosines in 12S mitochondrial rRNA. It is a key component of the mitochondrial transcription initiation complex, which includes TFAM and POLRMT, and is essential for basal transcription of mitochondrial DNA. TFB2M facilitates promoter opening and structural changes in POLRMT, independent of its methyltransferase activity, highlighting its dual role in mitochondrial gene expression. The protein is localized exclusively in the mitochondrion, underscoring its importance in mitochondrial function and energy metabolism.