C-terminal NH2. Active against S. aureus ATCC 25923 (MIC 3 uM), E. faecalis ATCC 29212 (MIC 10 uM), B. megaterium (2 uM), Gram-negative E. coli ATCC 25922 (MIC 10 uM), E. coli ATCC 35218 (MIC 10 uM), P. aeruginosa ATCC 27853 (MIC 20 uM), fungi C. albicans ATCC 90028 (MIC 16 uM), C. parapsilosis ATCC 22019 (MIC 31 uM), and S. cerevisiae (MIC 8 uM). 50% hemolysis at 25 uM. The peptide is helical in 40% TFE based on CD. 3D structure bound to SDS micelles is known with the three glycines on the polar surface (Biochemistry 2008; 47: 10513-25). Updated 3/2014. Temporin-SHa has Antibacterial, Antifungal activity. The source of Temporin-SHa is the skin of the Sahara frog Pelophylax (Rana) saharica originating from Tunisia.
Abbassi F, Oury B, Blasco T, et al. Isolation, characterization and molecular cloning of new temporins from the skin of the North African ranid Pelophylax saharica. Peptides. 2008;29(9):1526-33.