- 抗体类型:多克隆
- 抗体来源:兔
- 抗体应用:ELISA, WB, IP, IHC, IF, FC
- 特异性:Human, Mouse; other species not tested.
产品详情
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产品名称
STIM1 antibody
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抗体类型
多克隆
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抗体来源
兔
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抗体亚型
兔IgG
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抗体描述
STIM1 Rabbit Polyclonal antibody. Positive IP detected in Raji cells. Positive WB detected in Jurkat cells, BxPC-3 cells, HT-1080 cells, mouse liver tissue, NIH/3T3 cells, Raji cells. Positive FC detected in HepG2 cells. Positive IHC detected in human ovary tumor tissue, human lung cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 80-90 kDa
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抗体应用
ELISA, WB, IP, IHC, IF, FC
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应用推荐
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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特异性
Human, Mouse; other species not tested.
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蛋白别名
D11S4896E, GOK, STIM1, stromal interaction molecule 1
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制备方法
This antibody was obtained by immunization of STIM1 recombinant protein (Accession Number: NM_003156). Purification method: Antigen affinity purified.
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组分
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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储存方法
Store at -20℃. DO NOT ALIQUOT
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背景介绍
STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER–plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.The MW of STIM1 is about 80-90 kDa which may be due to the phosphorylation or glycosylation forms. Catalog#11565-1-AP is rabbit polyclonal antibody raised against the N-terminal of human STIM1.
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参考文献
- Hong JH, Li Q, Kim MS. Polarized but differential localization and recruitment of STIM1, Orai1 and TRPC channels in secretory cells. Traffic (Copenhagen, Denmark). 12(2):232-45. 2011.
- DebRoy A, Vogel SM, Soni D, Sundivakkam PC, Malik AB, Tiruppathi C. Cooperative signaling via transcription factors NF-κB and AP1/c-Fos mediates endothelial cell STIM1 expression and hyperpermeability in response to endotoxin. The Journal of biological chemistry. 289(35):24188-201. 2014.
- Wang Y, Chaudhari S, Ren Y, Ma R. Impairment of hepatic nuclear factor-4α binding to the Stim1 promoter contributes to high glucose-induced upregulation of STIM1 expression in glomerular mesangial cells. American journal of physiology. Renal physiology. 308(10):F1135-45. 2015.
- MacGlashan D. Expression profiling of human basophils: modulation by cytokines and secretagogues. PloS one. 10(5):e0126435. 2015.
- Zhang H, Sun AY, Kim JJ. STIM1-Ca2+ signaling modulates automaticity of the mouse sinoatrial node. Proceedings of the National Academy of Sciences of the United States of America. 112(41):E5618-27. 2015.
- Gruszczynska-Biegala J, Pomorski P, Wisniewska MB, Kuznicki J. Differential roles for STIM1 and STIM2 in store-operated calcium entry in rat neurons. PloS one. 6(4):e19285. 2011.
- Sundivakkam PC, Natarajan V, Malik AB, Tiruppathi C. Store-operated Ca2+ entry (SOCE) induced by protease-activated receptor-1 mediates STIM1 protein phosphorylation to inhibit SOCE in endothelial cells through AMP-activated protein kinase and p38β mitogen-activated protein kinase. The Journal of biological chemistry. 288(23):17030-41. 2013.
- Chaudhari S, Wu P, Wang Y. High glucose and diabetes enhanced store-operated Ca(2+) entry and increased expression of its signaling proteins in mesangial cells. American journal of physiology. Renal physiology. 306(9):F1069-80. 2014.