Recombinant Human Kinetochore protein Spc25 (hSpc25) fragment (amino acids 1-224) is a high-purity, stable protein designed for structural and biochemical studies. This fragment encompasses a computationally optimized soluble domain, ideal for investigating kinetochore assembly, microtubule interactions, and mitotic regulation.

The full-length Spc25 protein is a critical component of the NDC80 complex, essential for accurate chromosome segregation and spindle checkpoint activity during cell division. It localizes to the outer plate of the kinetochore from late prophase to anaphase, where it helps maintain kinetochore integrity and organizes microtubule-binding sites. The NDC80 complex, including Spc25, synergistically enhances microtubule affinity with the SKA1 complex, enabling tracking of depolymerizing microtubules and ensuring proper chromosome alignment and separation.