RFRP-1 is a peptide that binds to neuropeptide FF receptor 2 (NPFF 2 R) and has a significant impact on cardiovascular performance. It is found in cardiomyocytes and heart and can affect the Ca2+ transient and myocyte shortening. The myofilament is the primary downstream target of RFRP-1, and protein kinase C (PKC) plays a role in its influence on the Ca2+ transient. The study suggests that molecules targeting the RFRP-1 pathway may help address cardiovascular disease.