重组大鼠IL25蛋白

大鼠

人胚胎肾细胞（HEK293）

Val 17 - Ala 169

A DNA sequence encoding the rat IL25 (D3ZLB1) (Val17-Ala169) was expressed, fused with Fc region of human IgG1 at the N-terminus.

> 85 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized mouse IL17BR-His (Cat:502256) at 10 μg/mL (100 μl/well) can bind rat Fc-IL25 (Cat:500018). The EC₅₀ of rat Fc-IL25 is 0.12-0.27 μg/mL.

The recombinant rat IL25 comprises 413 amino acids and predicts a molecular mass of 46.1 kDa. The apparent molecular mass of the recombinant protein is approximately 49 kDa in SDS-PAGE under reducing conditions due to glycosylation.

RATTUS NORVEGICUS; RGD1561632; IL-17E; IL17E; UNQ3120; PRO10272

Protein Il25

Interleukin-25 (IL-25) is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. IL-25 is a member of the IL-17 family of cytokines. However, unlike the other members of this family, IL-25 promotes T helper (Th) 2 responses. IL-25 also regulates the development of autoimmune inflammation mediated by IL-17-producing T cells. IL-25 and IL-17, being members of the same cytokine family, play opposing roles in the pathogenesis of organ-specific autoimmunity. IL-25 promotes cell expansion and Th2 cytokine production when Th2 central memory cells are stimulated with thymic stromal lymphopoietin (TSLP)-activated dendritic cells (DCs), homeostatic cytokines, or T cell receptor for antigen triggering. Elevated expression of IL-25 and IL-25R transcripts was observed in asthmatic lung tissues and atopic dermatitis skin lesions, linking their possible roles with exacerbated allergic disorders. A plausible explanation that IL-25 produced by innate effector eosinophils and basophils may augment the allergic inflammation by enhancing the maintenance and functions of adaptive Th2 memory cells had been provided.

Lyophilized from sterile PBS, pH 7.4
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

< 1.0 EU per μg of the protein as determined by the LAL method

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.