重组人PCNA蛋白

人

杆状病毒 - 昆虫细胞

Met 1 - Ser 261

A DNA sequence encoding the mature form of human PCNA (P12004) (Met 1-Ser 261) was expressed, with a polyhistidine tag at the N-terminus.

> 90 % as determined by SDS-PAGE

The recombinant human PCNA consists of 280 amino acids and has a calculated molecular mass of 31 kDa. It migrates as an approximately 36 kDa band in SDS-PAGE under reducing conditions.

RP23-33N15.1; MGC8367; ATLD2

Proliferating cell nuclear antigen

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

Lyophilized from sterile 50mM Na3PO4, 300mM NaCl, 10% glycerol, pH 7.0, 2mM DTT
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

< 1.0 EU per μg of the protein as determined by the LAL method

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.