重组人PF4蛋白

人

大肠杆菌

Glu 32 - Ser 101

A DNA sequence encoding the human CXCL4 (NP_002610.1) (Glu 32-Ser 101) was expressed.

> 80 % as determined by SDS-PAGE

1. Measured by its ability to inhibit human bFGF / FGF2 dependent proliferation of Balb/c3T3 mouse fibroblasts. The ED₅₀ for this effect is typically 5-40 μg/ml.
2. Measured by its binding ability in a functional ELISA. Immobilized human CXCL4 at 20 μg/ml (100 μl/well) can bind biotinylated human VEGF165. The EC₅₀ of biotinylated human VEGF165 is 9.97 μg/ml.

The recombinant human CXCL4 comprises 71 amino acids with a predicted molecular mass of 7.8 kDa as estimated in SDS-PAGE under reducing conditions.

PF4; PF-4; MGC138298; SCYB4; CXCL4

Platelet factor 4

Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.

Lyophilized from sterile PBS, pH 7.4
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

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Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.