重组人NCF2蛋白

人

杆状病毒 - 昆虫细胞

Met 1 - Val 526

A DNA sequence encoding the human NCF2 (AAH01606.1) (Met1-Val526) was expressed and purified with two additional amino acids (Gly & Pro) at the N-terminus.

> 85 % as determined by SDS-PAGE

The secreted recombinant human NCF2 consists of 528 amino acids and predicts a molecular mass of 59.9 KDa. The apparent molecular mass of the protein is approximately 60 KDa in SDS-PAGE under reducing conditions due to glycosylation.

P67PHOX; RP1-127C7.5; NCF-2; NEUTROPHIL CYTOSOLIC FACTOR 2 (65KD; NEUTROPHIL CYTOSOLIC FACTOR 2; AUTOSOMAL 2; NADPH OXIDASE ACTIVATOR 2; AUTOSOMAL 2); 67 KDA NEUTROPHIL OXIDASE FACTOR; NEUTROPHIL CYTOSOL FACTOR 2; CHRONIC GRANULOMATOUS DISEASE; NOXA2; NEUTROPHIL NADPH OXIDASE FACTOR 2; NADPH OXIDASE SUBUNIT (67 KD); CHRONIC GRANULOMATOUS DISEASE; NCF2; NADPH OXIDASE SUBUNIT (67KDA); P67-PHOX

Neutrophil cytosol factor 2

NCF2, also known as NCF-2 and p67phox, is a subunit of the multi-protein NADPH oxidase complex. NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in NCF2 gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms.

Lyophilized from sterile 20mM Tris, 300mM NaCl, pH 8.0.
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

< 1.0 EU per μg of the protein as determined by the LAL method

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.