重组小鼠TNFRSF1B蛋白

小鼠

人胚胎肾细胞（HEK293）

Met 1 - Gly 258

A DNA sequence encoding the extracellular domain of mouse TNFRSF1B (NP_035740.2) (Met 1-Gly 258) was fused with a polyhistidine tag at the C-terminus.

> 97 % as determined by SDS-PAGE

Measured by its ability to inhibit the TNFα mediated cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.
The ED₅₀ for this effect is typically 1-3 μg/mL in the presence of 0.1 ng/mL of recombinant mouse TNFα.

The recombinant mouse TNFRSF1B consists of 247 amino acids and has a predicted molecular mass of 26.8 kDa. In SDS-PAGE under reducing conditions, rm TNFRSF1B migrates as an approximately 45 kDa band due to glycosylation.

P75 TNF RECEPTOR; TNF-R-II; TNFRII; P75; TNFR2; TNFRSF1B; TNFR-1; TBPII; TNFR-II; P75TNFR; TNF-R75; TNFBR; TNF-RII; TNFR80; TNF-R2; CD120B; P75 TNFR; TNFALPHA-R2; RP23-157J11.1; TNFR1B; TNF-ALPHAR2

Tumor necrosis factor receptor superfamily member 1B

Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B), also known as Tumor necrosis factor receptor 2 (TNFR2) or CD120b antigen, is a member of the tumor necrosis factor receptor superfamily. TNFR2/CD120b/TNFRSF1B is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. TNFR2/CD120b/TNFRSF1B is not a major contributing factor to the genetic risk of type 2 diabetes, its associated peripheral neuropathy and hypertension and related metabolic traits in North Indians. Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B) has been reported to be associated with SLE risk in Japanese populations. TNFR2/CD120b/TNFRSF1B serves as a receptor with high affinity for TNFSF2 and approximately 5-fold lower affinity for homotrimeric TNFSF1. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.

Lyophilized from sterile PBS, pH 7.4
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

< 1.0 EU per μg of the protein as determined by the LAL method

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.